Polarized sorting of Patched enables cytoneme-mediated Hedgehog reception in the Drosophila wing disc.
EMBO J
; 39(11): e103629, 2020 06 02.
Article
en En
| MEDLINE
| ID: mdl-32311148
Hedgehog (Hh) signal molecules play a fundamental role in development, adult stem cell maintenance and cancer. Hh can signal at a distance, and we have proposed that its graded distribution across Drosophila epithelia is mediated by filopodia-like structures called cytonemes. Hh reception by Patched (Ptc) happens at discrete sites along presenting and receiving cytonemes, reminiscent of synaptic processes. Here, we show that a vesicle fusion mechanism mediated by SNARE proteins is required for Ptc placement at contact sites. Transport of Ptc to these sites requires multivesicular bodies (MVBs) formation via ESCRT machinery, in a manner different to that regulating Ptc/Hh lysosomal degradation after reception. These MVBs include extracellular vesicle (EV) markers and, accordingly, Ptc is detected in the purified exosomal fraction from cultured cells. Blockage of Ptc trafficking and fusion to basolateral membranes result in low levels of Ptc presentation for reception, causing an extended and flattened Hh gradient.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Alas de Animales
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Receptores de Superficie Celular
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Proteínas de Drosophila
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Proteínas SNARE
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Proteínas Hedgehog
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Complejos de Clasificación Endosomal Requeridos para el Transporte
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Discos Imaginales
Límite:
Animals
Idioma:
En
Revista:
EMBO J
Año:
2020
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Reino Unido