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ALDH1A1+ ovarian cancer stem cells co-expressing surface markers CD24, EPHA1 and CD9 form tumours in vivo.
Nagare, Rohit P; Sneha, Smarakan; Krishnapriya, Syama; Ramachandran, Balaji; Murhekar, Kanchan; Vasudevan, Sekar; Shabna, Aboo; Ganesan, Trivadi S.
Afiliación
  • Nagare RP; Laboratory for Cancer Biology, Cancer Institute (WIA), Chennai, Tamilnadu, India.
  • Sneha S; Laboratory for Cancer Biology, Cancer Institute (WIA), Chennai, Tamilnadu, India.
  • Krishnapriya S; Laboratory for Cancer Biology, Cancer Institute (WIA), Chennai, Tamilnadu, India.
  • Ramachandran B; Department of Molecular Oncology, Cancer Institute (WIA), Chennai, India.
  • Murhekar K; Department of Pathology, Cancer Institute (WIA), Chennai, Tamilnadu, India.
  • Vasudevan S; Laboratory for Cancer Biology, Cancer Institute (WIA), Chennai, Tamilnadu, India.
  • Shabna A; Laboratory for Cancer Biology, Cancer Institute (WIA), Chennai, Tamilnadu, India.
  • Ganesan TS; Laboratory for Cancer Biology, Cancer Institute (WIA), Chennai, Tamilnadu, India; Department of Medical Oncology and Clinical Research, Cancer Institute (WIA), Chennai, Tamilnadu, India. Electronic address: tsganesan@cancerinstitutewia.org.
Exp Cell Res ; 392(1): 112009, 2020 07 01.
Article en En | MEDLINE | ID: mdl-32305326
One of the reasons for recurrence following treatment of high grade serous ovarian carcinoma (HGSOC) is the persistence of residual cancer stem cells (CSCs). There has been variability between laboratories in the identification of CSC markers for HGSOC. We have identified new surface markers (CD24, CD9 and EPHA1) in addition to those previously known (CD44, CD117 and CD133) using a bioinformatics approach. The expression of these surface markers was evaluated in ovarian cancer cell lines, primary malignant cells (PMCs), normal ovary and HGSOC. There was no preferential expression of any of the markers or a combination. All the markers were expressed at variable levels in ovarian cancer cell lines and PMCs. Only CD117 and CD9 were expressed in the normal ovarian surface epithelium and fallopian tube. Both ALDEFLUOR (ALDH1A1) and side population assays identified a small proportion of cells (<3%) separately that did not overlap with little variability in cell lines and PMCs. All surface markers were co-expressed in ALDH1A1+ cells without preference for one combination. The cell cycle analysis of ALDH1A1+ cells alone revealed that majority of them reside in G0/G1 phase of cell cycle. Further separation of G0 and G1 phases showed that ALDH1A1+ cells reside in G1 phase of the cell cycle. Xenograft assays showed that the combinations of ALDH1A1 + cells co-expressing CD9, CD24 or EPHA1 were more tumorigenic and aggressive with respect to ALDH1A1-cells. These data suggest that a combined approach could be more useful in identifying CSCs in HGSOC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Ováricas / Células Madre Neoplásicas / Biomarcadores de Tumor / Cistadenocarcinoma Seroso / Retinal-Deshidrogenasa / Familia de Aldehído Deshidrogenasa 1 Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Exp Cell Res Año: 2020 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Ováricas / Células Madre Neoplásicas / Biomarcadores de Tumor / Cistadenocarcinoma Seroso / Retinal-Deshidrogenasa / Familia de Aldehído Deshidrogenasa 1 Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Exp Cell Res Año: 2020 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos