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Molecular profiling of allergen-specific antibody responses may enhance success of specific immunotherapy.
Rodríguez-Domínguez, Azahara; Berings, Margot; Rohrbach, Alexander; Huang, Huey-Jy; Curin, Mirela; Gevaert, Philippe; Matricardi, Paolo M; Valenta, Rudolf; Vrtala, Susanne.
Afiliación
  • Rodríguez-Domínguez A; Department of Pathophysiology and Allergy Research, Division of Immunopathology, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna and Krems, Austria.
  • Berings M; Upper Airways Research Laboratory, Ghent University, Ghent, Belgium.
  • Rohrbach A; Department of Pediatric Pulmonology, Immunology and Intensive Care Medicine, Charité-University Medicine Berlin, Berlin, Germany.
  • Huang HJ; Department of Pathophysiology and Allergy Research, Division of Immunopathology, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna and Krems, Austria.
  • Curin M; Department of Pathophysiology and Allergy Research, Division of Immunopathology, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna and Krems, Austria.
  • Gevaert P; Upper Airways Research Laboratory, Ghent University, Ghent, Belgium.
  • Matricardi PM; Department of Pediatric Pulmonology, Immunology and Intensive Care Medicine, Charité-University Medicine Berlin, Berlin, Germany.
  • Valenta R; Department of Pathophysiology and Allergy Research, Division of Immunopathology, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna and Krems, Austria; NRC Institute of Immunology, Federal Biomedical Agency of Russia, Moscow; Department of Clinical Immunolog
  • Vrtala S; Department of Pathophysiology and Allergy Research, Division of Immunopathology, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna and Krems, Austria. Electronic address: susanne.vrtala@meduniwien.ac.at.
J Allergy Clin Immunol ; 146(5): 1097-1108, 2020 11.
Article en En | MEDLINE | ID: mdl-32298697
BACKGROUND: House dust mites (HDMs) are among the most important allergen sources containing many different allergenic molecules. Analysis of patients from a double-blind, placebo-controlled allergen-specific immunotherapy (AIT) study indicated that patients may benefit from AIT to different extents depending on their molecular sensitization profiles. OBJECTIVE: Our aim was to investigate in a real-life setting whether stratification of patients with HDM allergy according to molecular analysis may enhance AIT success. METHODS: Serum and nasal secretion samples from patients with HDM allergy (n = 24) (at baseline, 7, 15, 33, and 52 weeks) who had received 1 year of treatment with a well-defined subcutaneous AIT form (Alutard SQ 510) were tested for IgE and IgG reactivity to 15 microarrayed HDM allergen molecules with ImmunoCAP Immuno-solid-phase Allergen Chip technology. IgG subclass levels to allergens and peptides were determined by ELISA, and IgG blocking was assessed by basophil activation. In vitro parameters were related to reduction of symptoms determined by combined symptom medication score and visual analog scale score. RESULTS: Alutard SQ 510 induced protective IgG mainly against Dermatophagoides pteronyssinus (Der p) 1 and Der p 2 and to a lesser extent to Der p 23, but not to the other important allergens such as Der p 5, Der p 7, and Der p 21, showing better clinical efficacy in patients sensitized only to Der p 1 and/or Der p 2 as compared with patients having additional IgE specificities. CONCLUSION: Stratification of patients with HDM allergy according to molecular sensitization profiles and molecular monitoring of AIT-induced IgG responses may enhance the success of AIT.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoglobulina E / Inmunoglobulina G / Cisteína Endopeptidasas / Extractos Vegetales / Desensibilización Inmunológica / Antígenos Dermatofagoides / Proteínas de Artrópodos / Hipersensibilidad Tipo de estudio: Clinical_trials Límite: Adult / Animals / Female / Humans / Male Idioma: En Revista: J Allergy Clin Immunol Año: 2020 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoglobulina E / Inmunoglobulina G / Cisteína Endopeptidasas / Extractos Vegetales / Desensibilización Inmunológica / Antígenos Dermatofagoides / Proteínas de Artrópodos / Hipersensibilidad Tipo de estudio: Clinical_trials Límite: Adult / Animals / Female / Humans / Male Idioma: En Revista: J Allergy Clin Immunol Año: 2020 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Estados Unidos