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Glycan characterization of pregnancy-specific glycoprotein 1 and its identification as a novel Galectin-1 ligand.
Mendoza, Mirian; Lu, Dongli; Ballesteros, Angela; Blois, Sandra M; Abernathy, Kelsey; Feng, Chiguang; Dimitroff, Charles J; Zmuda, Jonathan; Panico, Maria; Dell, Anne; Vasta, Gerardo R; Haslam, Stuart M; Dveksler, Gabriela.
Afiliación
  • Mendoza M; Department of Pathology, Uniformed Services University, 4301 Jones Bridge Rd, Bethesda, MD 20814, USA.
  • Lu D; Department of Life Sciences, Imperial College London, South Kensington, London SW7 2BU, UK.
  • Ballesteros A; Molecular Physiology and Biophysics Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
  • Blois SM; Experimental and Clinical Research Center, Charité Campus Buch, Lindenberger Weg 80, 13125 Berlin, Germany.
  • Abernathy K; Charité- Universitätsmedizin Berlin, Institute for Medical Immunology, Augustenburger Platz 1, 13353 Berlin, Germany.
  • Feng C; Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland School of Medicine, 655 W Baltimore St S, Baltimore, MD 21201, USA.
  • Dimitroff CJ; Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland School of Medicine, 655 W Baltimore St S, Baltimore, MD 21201, USA.
  • Zmuda J; Translational Medicine, Translational Glycobiology Institute, FIU, Herbert Wertheim College of Medicine, Florida International University, 11200 SW 8th St, Miami, FL 33199, USA.
  • Panico M; Biosciences Division, Thermo Fisher Scientific, 7335 Executive Way, Frederick MD 21704, USA.
  • Dell A; Department of Life Sciences, Imperial College London, South Kensington, London SW7 2BU, UK.
  • Vasta GR; Department of Life Sciences, Imperial College London, South Kensington, London SW7 2BU, UK.
  • Haslam SM; Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland School of Medicine, 655 W Baltimore St S, Baltimore, MD 21201, USA.
  • Dveksler G; Department of Life Sciences, Imperial College London, South Kensington, London SW7 2BU, UK.
Glycobiology ; 30(11): 895-909, 2020 10 21.
Article en En | MEDLINE | ID: mdl-32280962
Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester. PSG1 has seven potential N-linked glycosylation sites across its four domains. We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites. Using different techniques, we explored whether PSG1 can bind to galectin-1 (Gal-1) as these two proteins were previously shown to participate in processes required for a successful pregnancy. We confirmed that PSG1 binds to Gal-1 in a carbohydrate-dependent manner with an affinity of the interaction of 0.13 µM. In addition, we determined that out of the three N-glycosylation-carrying domains, only the N and A2 domains of recombinant PSG1 interact with Gal-1. Lastly, we observed that the interaction between PSG1 and Gal-1 protects this lectin from oxidative inactivation and that PSG1 competes the ability of Gal-1 to bind to some but not all of its glycoprotein ligands.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Glicoproteínas beta 1 Específicas del Embarazo / Galectina 1 Tipo de estudio: Diagnostic_studies Límite: Female / Humans / Pregnancy Idioma: En Revista: Glycobiology Asunto de la revista: BIOQUIMICA Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Glicoproteínas beta 1 Específicas del Embarazo / Galectina 1 Tipo de estudio: Diagnostic_studies Límite: Female / Humans / Pregnancy Idioma: En Revista: Glycobiology Asunto de la revista: BIOQUIMICA Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido