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Boron doped silver-copper alloy nanoparticle targeting intracellular S. aureus in bone cells.
Abdulrehman, Tahir; Qadri, Shahnaz; Skariah, Sini; Sultan, Ali; Mansour, Said; Azzi, Jamil; Haik, Yousef.
Afiliación
  • Abdulrehman T; College of Health and Life Sciences, Hamad Bin Khalifa University, Doha, Qatar.
  • Qadri S; College of Science and Engineering, Hamad Bin Khalifa University, Doha, Qatar.
  • Skariah S; Weil Cornell Medicine-Qatar, Education City, Doha, Qatar.
  • Sultan A; Weil Cornell Medicine-Qatar, Education City, Doha, Qatar.
  • Mansour S; Qatar Environment and Energy Research Institute (QEERI), Hamad Bin Khalifa University, Doha, Qatar.
  • Azzi J; Brigham and Women's Hospital, Harvard Medical School, Boston, United States of America.
  • Haik Y; College of Science and Engineering, Hamad Bin Khalifa University, Doha, Qatar.
PLoS One ; 15(4): e0231276, 2020.
Article en En | MEDLINE | ID: mdl-32275737
OBJECTIVES: Alloyed metallic nanoparticles of silver and copper are effective against intracellular infection. However, systemic toxicity may arise due to the non-specific delivery of the nanoparticles. In addressing the issue, this study deals with the targeting of silver-copper-boron (ACB) nanoparticles to infected osteoblasts, which could decrease systemic toxicity and form the basis of targeting specific markers expressed in bone infections. METHODS: ACB nanoparticles were synthesized and conjugated to the Cadherin-11 antibody (OBAb). The effect of targeting nanoparticles against extracellular and intracellular S. aureus was determined by enumeration of bacterial growth. The binding of the targeting nanoparticles to infected osteoblasts as well as the visualization of live/dead bacteria due to treatment was carried out using fluorescence microscopy. MTT assay was used to determine the viability of osteoblasts with different concentrations of the nanoparticles. RESULTS: The ACB nanoparticles conjugated to OBAb (ACB-OBAb) were effective against extracellular S. aureus. The ACB-OBAb nanoparticles showed a 1.32 log reduction of intracellular S. aureus at a concentration of 1mg/L. The ACB-OBAb nanoparticles were able to bind to the infected osteoblast and showed toxicity to osteoblasts at levels ≥20mg/L. Also, the percentage of silver, copper, and boron in the nanoparticles determined the effectiveness of their antibacterial activity. CONCLUSION: The ACB-OBAb nanoparticles were able to target the osteoblasts and demonstrated significant antibacterial activity against intracellular S. aureus. Targeting shows promise as a strategy to target specific markers expressed on infected osteoblasts for efficient nanoparticle delivery, and further animal studies are recommended to test its efficacy in vivo.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plata / Staphylococcus aureus / Huesos / Boro / Cobre / Espacio Intracelular / Aleaciones / Nanopartículas del Metal Límite: Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2020 Tipo del documento: Article País de afiliación: Qatar Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plata / Staphylococcus aureus / Huesos / Boro / Cobre / Espacio Intracelular / Aleaciones / Nanopartículas del Metal Límite: Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2020 Tipo del documento: Article País de afiliación: Qatar Pais de publicación: Estados Unidos