On-the-fly rapid immunoassay for neonatal sepsis diagnosis: C-reactive protein accurate determination using magnetic graphene-based micromotors.
Biosens Bioelectron
; 158: 112156, 2020 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-32275206
Based on the exceptional and new opened biosensing possibilities of self-propelled micromotors, a micromotor-based immunoassay (MIm) has smartly been designed for C-reactive protein (CRP) determination in plasma of preterm infants with sepsis suspicion. The design of the micromotors involved the electrosynthesis of a carbon-based outer layer (for antibody functionalization), an intermediate Ni layer (for magnetic guidance and stopped flow operations) and PtNPs inner catalytic layer (for catalytic bubble propulsion). Micromotors biofunctionalization on the outer layer (using carbon black (CB), reduced graphene oxide (rGO) and multi-walled carbon nanotubes (MWCNTs), and biocompatible propulsion capabilities, were carefully studied. Magnetic rGO/Ni/PtNPs micromotors exhibited the most efficient and reproducible (CV = 9%) anti-CRP functionalization, controlled stopped-flow operations as well as efficient bubble propulsion (1% H2O2, 1,5% NaCh, speed 140 µm s-1). Analytical performance of MIm was excellent, allowing the direct (without dilution), sensitive (LOD = 0.80 µg/mL), and accurate CRP determination (Er = 1%) in hardly available preterm babies' plasma samples with suspected sepsis using very low volumes (<10 µL) and in just 5 min of on-the-fly bioassay. Overall, the results obtained allowed the fast and reliable sepsis diagnostics in preterm babies' individuals with suspected sepsis, not only proving the usefulness of the approach as its potential utilization as point-of-care device for clinical analysis but drawing new horizons in extremely low sample volumes-based diagnostics.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteína C-Reactiva
/
Inmunoensayo
/
Técnicas Biosensibles
/
Nanotubos de Carbono
/
Sepsis Neonatal
/
Grafito
Tipo de estudio:
Diagnostic_studies
/
Etiology_studies
/
Guideline
Límite:
Humans
/
Newborn
Idioma:
En
Revista:
Biosens Bioelectron
Asunto de la revista:
BIOTECNOLOGIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Reino Unido