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Probing lasting cryoinjuries to oocyte-embryo transcriptome.
Eroglu, Binnur; Szurek, Edyta A; Schall, Peter; Latham, Keith E; Eroglu, Ali.
Afiliación
  • Eroglu B; Department of Neuroscience and Regenerative Medicine, Medical College of Georgia/Augusta University, Augusta, GA, United States of America.
  • Szurek EA; Department of Neuroscience and Regenerative Medicine, Medical College of Georgia/Augusta University, Augusta, GA, United States of America.
  • Schall P; Department of Obstetrics, Gynecology and Reproductive Biology, College of Agriculture & Natural Resources/Michigan State University, East Lansing, MI, United States of America.
  • Latham KE; Department of Obstetrics, Gynecology and Reproductive Biology, College of Agriculture & Natural Resources/Michigan State University, East Lansing, MI, United States of America.
  • Eroglu A; Department of Neuroscience and Regenerative Medicine, Medical College of Georgia/Augusta University, Augusta, GA, United States of America.
PLoS One ; 15(4): e0231108, 2020.
Article en En | MEDLINE | ID: mdl-32251418
Clinical applications of oocytes cryopreservation include preservation of future fertility of young cancer patients, substitution of embryo freezing to avoid associated legal and ethical issues, and delaying childbearing years. While the outcome of oocyte cryopreservation has recently been improved, currently used vitrification method still suffer from increased biosafety risk and handling issues while slow freezing techniques yield overall low success. Understanding better the mechanism of cryopreservation-induced injuries may lead to development of more reliable and safe methods for oocyte cryopreservation. Using the mouse model, a microarray study was conducted on oocyte cryopreservation to identify cryoinjuries to transcriptionally active genome. To this end, metaphase II (MII) oocytes were subjected to standard slow freezing, and then analyzed at the four-cell stage after embryonic genome activation. Non-frozen four-cell embryos served as controls. Differentially expressed genes were identified and validated using RT-PCR. Embryos produced from the cryopreserved oocytes displayed 200 upregulated and 105 downregulated genes, associated with the regulation of mitochondrial function, protein ubiquitination and maintenance, cellular response to stress and oxidative states, fatty acid and lipid regulation/metabolism, and cell cycle maintenance. These findings reveal previously unrecognized effects of standard slow oocyte freezing on embryonic gene expression, which can be used to guide improvement of oocyte cryopreservation methods.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Criopreservación / Embrión de Mamíferos / Transcriptoma / Congelación Tipo de estudio: Prognostic_studies Aspecto: Ethics Límite: Animals / Female / Humans / Male Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Criopreservación / Embrión de Mamíferos / Transcriptoma / Congelación Tipo de estudio: Prognostic_studies Aspecto: Ethics Límite: Animals / Female / Humans / Male Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos