Your browser doesn't support javascript.
loading
In vitro assessment of potential probiotic characteristics of indigenous Lactococcus lactis and Weissella oryzae isolates from rainbow trout (Oncorhynchus mykiss Walbaum).
Mortezaei, F; Royan, M; Allaf Noveirian, H; Babakhani, A; Alaie Kordghashlaghi, H; Balcázar, J L.
Afiliación
  • Mortezaei F; Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran.
  • Royan M; North Region Branch, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran.
  • Allaf Noveirian H; Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran.
  • Babakhani A; Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran.
  • Alaie Kordghashlaghi H; North Region Branch, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran.
  • Balcázar JL; Catalan Institute for Water Research (ICRA), Girona, Spain.
J Appl Microbiol ; 129(4): 1004-1019, 2020 Oct.
Article en En | MEDLINE | ID: mdl-32248610
AIM: The objective of this study was to evaluate the probiotic potential of lactic acid bacteria (LAB) isolated from the intestinal ecosystem of rainbow trout. METHODS AND RESULTS: Among LAB isolates, 10 of them were selected and screened for resistance to acid and bile salts, pancreatin, sodium chloride and temperature, hydrophobicity, growth profile and antimicrobial activity against fish pathogens. Then, biosafety assessments were investigated. Selected LAB tolerated to gastrointestinal physiological conditions, pancreatin and a range of sodium chloride and temperature. They also exhibited hydrophobicity and showed antagonistic activity against Streptococcus iniae and Yersinia ruckeri. Results of 16S rRNA gene sequencing showed that selected LAB belonged to the Lactococcus lactis (n = 5) and Weissella oryzae (n = 5) species. They exhibited no ß-haemolytic activity, while six selected LAB were resistant to some antibiotics. None of them harboured virulence factors. CONCLUSIONS: This study revealed probiotic characteristics of indigenous LAB isolated from the intestinal ecosystem of rainbow trout. However, further studies are required to confirm the effectiveness of these isolates as probiotics in aquaculture. SIGNIFICANCE AND IMPACT OF THE STUDY: To the best of our knowledge, for the first time, the presence of probiotic candidates belonging to W. Oryzae was confirmed in fish intestinal microbiota.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lactococcus lactis / Oncorhynchus mykiss / Probióticos / Weissella Límite: Animals Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lactococcus lactis / Oncorhynchus mykiss / Probióticos / Weissella Límite: Animals Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Reino Unido