Potential Antioxidant and Enzyme Inhibitory Effects of Nanoliposomal Formulation Prepared from Salvia aramiensis Rech. f. Extract.
Antioxidants (Basel)
; 9(4)2020 Apr 01.
Article
en En
| MEDLINE
| ID: mdl-32244734
Salvia aramiensis Rech. f. is a species that grows only in Hatay, Turkey and is used as a traditional stomachic tea. Neither the chemical composition nor the potential bioactivity of the plant has been investigated before. Antioxidant activity (1,1-Diphenyl-2-picrylhydrazyl Radical (DPPHâ) and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS+â) radical scavenging and ß-carotene/linoleic acid co-oxidation) of 70% methanol, 70% ethanol extracts, and 2% infusion obtained from S. aramiensis aerial parts were determined. The effect of 70% methanol extract on collagenase and elastase enzyme inhibition and its chemical composition via chromatographic methods (LC-MS/MS and HPLC) were analyzed. Nanoliposomes were developed with 70% methanol extract, were characterized, and were evaluated. The key parameters for the most active 70% methanol extract included the following DPPHâ¢EC50: 28.4 µg/mL, Trolox equivalent antioxidant capacity (TEAC)/ABTS: 1.77 ± 0.09 mmol/L/Trolox. Furthermore 70% methanol extract showed more than 50% inhibition on collagenase and elastase enzymes at all the concentrations. The main component of the extract, rich in phenolic compounds, has been identified as rosmarinic acid; 83.7 µg/mL extract was released from the nanoliposomal formulation. The extract and its formulation are found to be nontoxic on the L929 fibroblast cell line. This study successfully developed a long-term antioxidant and enzyme inhibitory formulation containing S. aramiensis, which has been used safely among the public for years.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Antioxidants (Basel)
Año:
2020
Tipo del documento:
Article
País de afiliación:
Turquía
Pais de publicación:
Suiza