Your browser doesn't support javascript.
loading
Nanoformulated Single-Stranded RNA-Based Adjuvant with a Coordinative Amphiphile as an Effective Stabilizer: Inducing Humoral Immune Response by Activation of Antigen-Presenting Cells.
Park, Hyo-Jung; Bang, Eun-Kyoung; Hong, Jung Joo; Lee, Sang-Myeong; Ko, Hae Li; Kwak, Hye Won; Park, Hyelim; Kang, Kyung Won; Kim, Rhoon-Ho; Ryu, Seung Rok; Kim, Green; Oh, Hanseul; Kim, Hye-Jung; Lee, Kyuri; Kim, Minjeong; Kim, Soo Young; Kim, Jae-Ouk; El-Baz, Karim; Lee, Hyukjin; Song, Manki; Jeong, Dae Gwin; Keum, Gyochang; Nam, Jae-Hwan.
Afiliación
  • Park HJ; Department of Biotechnology, The Catholic University of Korea, Bucheon, 14662, Republic of Korea.
  • Bang EK; Center for Neuro-Medicine, Brain Science Institute, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea.
  • Hong JJ; National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, 28116, Republic of Korea.
  • Lee SM; Division of Biotechnology, College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan, 54596, Republic of Korea.
  • Ko HL; Korea Zoonosis Research Institute, Jeonbuk National University, Iksan, 54531, Republic of Korea.
  • Kwak HW; Department of Biotechnology, The Catholic University of Korea, Bucheon, 14662, Republic of Korea.
  • Park H; Present address: Scripps Korea Antibody Institute, Chuncheon, 24341, Republic of Korea.
  • Kang KW; Department of Biotechnology, The Catholic University of Korea, Bucheon, 14662, Republic of Korea.
  • Kim RH; Department of Biotechnology, The Catholic University of Korea, Bucheon, 14662, Republic of Korea.
  • Ryu SR; Division of Biotechnology, College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan, 54596, Republic of Korea.
  • Kim G; Department of Biotechnology, The Catholic University of Korea, Bucheon, 14662, Republic of Korea.
  • Oh H; Division of Biotechnology, College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan, 54596, Republic of Korea.
  • Kim HJ; National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, 28116, Republic of Korea.
  • Lee K; National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, 28116, Republic of Korea.
  • Kim M; Department of Biotechnology, The Catholic University of Korea, Bucheon, 14662, Republic of Korea.
  • Kim SY; College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 03760, Republic of Korea.
  • Kim JO; College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 03760, Republic of Korea.
  • El-Baz K; College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 03760, Republic of Korea.
  • Lee H; Clinical Research Lab, International Vaccine Institute, Seoul National, University Research Park, Seoul, 08826, Republic of Korea.
  • Song M; Center for Neuro-Medicine, Brain Science Institute, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea.
  • Jeong DG; College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 03760, Republic of Korea.
  • Keum G; Clinical Research Lab, International Vaccine Institute, Seoul National, University Research Park, Seoul, 08826, Republic of Korea.
  • Nam JH; Infectious Diseases Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Angew Chem Int Ed Engl ; 59(28): 11540-11549, 2020 07 06.
Article en En | MEDLINE | ID: mdl-32239636
As agonists of TLR7/8, single-stranded RNAs (ssRNAs) are safe and promising adjuvants that do not cause off-target effects or innate immune overactivation. However, low stability prevents them from mounting sufficient immune responses. This study evaluates the adjuvant effects of ssRNA derived from the cricket paralysis virus intergenic region internal ribosome entry site, formulated as nanoparticles with a coordinative amphiphile, containing a zinc/dipicolylamine complex moiety as a coordinative phosphate binder, as a stabilizer for RNA-based adjuvants. The nanoformulated ssRNA adjuvant was resistant to enzymatic degradation in vitro and in vivo, and that with a coordinative amphiphile bearing an oleyl group (CA-O) was approximately 100 nm, promoted effective recognition, and improved activation of antigen-presenting cells, leading to better induction of neutralizing antibodies following single immunization. Hence, CA-O may increase the efficacy of ssRNA-based adjuvants, proving useful to meet the urgent need for vaccines during pathogen outbreaks.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN / Adyuvantes Inmunológicos / Nanotecnología / Composición de Medicamentos / Inmunidad Humoral / Células Presentadoras de Antígenos Límite: Animals / Humans Idioma: En Revista: Angew Chem Int Ed Engl Año: 2020 Tipo del documento: Article Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN / Adyuvantes Inmunológicos / Nanotecnología / Composición de Medicamentos / Inmunidad Humoral / Células Presentadoras de Antígenos Límite: Animals / Humans Idioma: En Revista: Angew Chem Int Ed Engl Año: 2020 Tipo del documento: Article Pais de publicación: Alemania