Labeling Antibodies Using Europium.
Cold Spring Harb Protoc
; 2020(4): 099325, 2020 04 01.
Article
en En
| MEDLINE
| ID: mdl-32238595
There are many uses for antibodies labeled with metal ions. Most of these methods involve first attaching a metal chelator to the antibody molecule. This is achieved using standard cross-linking chemistry and then adding the desired metal at appropriate concentration and pH. The method described here outlines a basic procedure for creating a lanthanide conjugate. Lanthanide conjugates are used for proximity assays, as MRI contrast agents, or for mass cytometry experiments. Different metals and chelators can be substituted, but the basic procedures are similar.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Coloración y Etiquetado
/
Quelantes
/
Europio
/
Anticuerpos
Idioma:
En
Revista:
Cold Spring Harb Protoc
Año:
2020
Tipo del documento:
Article
Pais de publicación:
Estados Unidos