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The protective role of metformin in autophagic status in peripheral blood mononuclear cells of type 2 diabetic patients.
Bhattacharya, Debalina; Dutta, Moumita; Mukhopadhyay, Mainak; Bhattacharyya, Maitree; Chowdhury, Subhankar; Karmakar, Parimal.
Afiliación
  • Bhattacharya D; Department of Microbiology, Maulana Azad College, Kolkata, West Bengal, India.
  • Dutta M; Division of Electron Microscopy, ICMR-NICED, Kolkata, West Bengal, India.
  • Mukhopadhyay M; Department of Biotechnology, JIS University, Agarpara, Kolkata, West Bengal, India.
  • Bhattacharyya M; Department of Biochemistry, University of Calcutta, Kolkata, West Bengal, India.
  • Chowdhury S; Department of Endocrinology & Metabolism, Institute of Postgraduate Medical Education and Research, Kolkata, West Bengal, India.
  • Karmakar P; Department of Life Science and Biotechnology, Jadavpur University, Kolkata, West Bengal, India.
Cell Biol Int ; 44(8): 1628-1639, 2020 Aug.
Article en En | MEDLINE | ID: mdl-32237184
Autophagy plays an important role in the pathophysiology of type 2 diabetes (T2D). Metformin is the most common antidiabetic drug. The main objective of this study was to explore the molecular mechanism of metformin in starvation-induced autophagy in peripheral blood mononuclear cells (PBMCs) of type 2 diabetic patients. PBMCs were isolated from 10 diabetic patients and 7 non-diabetic healthy volunteers. The autophagic puncta and markers were measured with the help of monodansylcadaverine staining and western blot. Additionally, transmission electron microscopy was also performed. No significant changes were observed in the initial autophagy marker protein levels in PBMCs of T2D after metformin treatment though diabetic PBMCs showed a high level of phospho-mammalian target of rapamycin, p62 and reduced expression of phospho-AMP-activated protein kinase and lysosomal membrane-associated protein 2, indicating a defect in autophagy. Also, induction of autophagy by tunicamycin resulted in apoptosis in diabetic PBMCs as observed by caspase-3 cleavage and reduced expression of Bcl2. Inhibition of autophagy by bafilomycin rendered consistent expression of p62 indicating a defect in the final process of autophagy. Further, electron microscopic studies also confirmed massive vacuole overload and a sign of apoptotic cell death in PBMCs of diabetic patients, whereas metformin treatment reduced the number of autophagic vacuoles perhaps by lysosomal fusion. Thus, our results indicate that defective autophagy in T2D is associated with the fusion process of lysosomes which could be overcome by metformin.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Autofagia / Leucocitos Mononucleares / Diabetes Mellitus Tipo 2 / Hipoglucemiantes / Metformina Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Cell Biol Int Año: 2020 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Autofagia / Leucocitos Mononucleares / Diabetes Mellitus Tipo 2 / Hipoglucemiantes / Metformina Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Cell Biol Int Año: 2020 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido