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Analysis of Intracellular Magnesium and Mineral Depositions during Osteogenic Commitment of 3D Cultured Saos2 Cells.
Picone, Giovanna; Cappadone, Concettina; Pasini, Alice; Lovecchio, Joseph; Cortesi, Marilisa; Farruggia, Giovanna; Lombardo, Marco; Gianoncelli, Alessandra; Mancini, Lucia; Ralf H, Menk; Donato, Sandro; Giordano, Emanuele; Malucelli, Emil; Iotti, Stefano.
Afiliación
  • Picone G; Department of Pharmacy and Biotechnology, University of Bologna, 33-40126 Bologna, Italy.
  • Cappadone C; Department of Pharmacy and Biotechnology, University of Bologna, 33-40126 Bologna, Italy.
  • Pasini A; Department of Electrical, Electronic and Information Engineering "Guglielmo Marconi" (DEI), University of Bologna 50, 47522 Cesena, Italy.
  • Lovecchio J; Department of Electrical, Electronic and Information Engineering "Guglielmo Marconi" (DEI), University of Bologna 50, 47522 Cesena, Italy.
  • Cortesi M; Department of Electrical, Electronic and Information Engineering "Guglielmo Marconi" (DEI), University of Bologna 50, 47522 Cesena, Italy.
  • Farruggia G; Department of Pharmacy and Biotechnology, University of Bologna, 33-40126 Bologna, Italy.
  • Lombardo M; National Institute of Biostructures and Biosystems (NIBB), 00136 Rome, Italy.
  • Gianoncelli A; Department of Chemistry "G. Ciamician", Alma Mater Studiorum-Università di Bologna, via Selmi 2, I-40126 Bologna, Italy.
  • Mancini L; Elettra-Sincrotrone Trieste S.C.p.A., Trieste, 34149 Basovizza, Italy.
  • Ralf H M; Elettra-Sincrotrone Trieste S.C.p.A., Trieste, 34149 Basovizza, Italy.
  • Donato S; Elettra-Sincrotrone Trieste S.C.p.A., Trieste, 34149 Basovizza, Italy.
  • Giordano E; INFN section of Trieste, 2-34127 Trieste, Italy.
  • Malucelli E; Department of Medical Imaging, University of Saskatchewan, Saskatoon, SK S7N 5A2, Canada.
  • Iotti S; Elettra-Sincrotrone Trieste S.C.p.A., Trieste, 34149 Basovizza, Italy.
Int J Mol Sci ; 21(7)2020 Mar 30.
Article en En | MEDLINE | ID: mdl-32235449
In this study, we explore the behaviour of intracellular magnesium during bone phenotype modulation in a 3D cell model built to mimic osteogenesis. In addition, we measured the amount of magnesium in the mineral depositions generated during osteogenic induction. A two-fold increase of intracellular magnesium content was found, both at three and seven days from the induction of differentiation. By X-ray microscopy, we characterized the morphology and chemical composition of the mineral depositions secreted by 3D cultured differentiated cells finding a marked co-localization of Mg with P at seven days of differentiation. This is the first experimental evidence on the presence of Mg in the mineral depositions generated during biomineralization, suggesting that Mg incorporation occurs during the bone forming process. In conclusion, this study on the one hand attests to an evident involvement of Mg in the process of cell differentiation, and, on the other hand, indicates that its multifaceted role needs further investigation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Fósforo / Magnesio Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2020 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Fósforo / Magnesio Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2020 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza