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Investigation of LuxS-mediated quorum sensing in Klebsiella pneumoniae.
Chen, Lijiang; Wilksch, Jonathan J; Liu, Haiyang; Zhang, Xiaoxiao; Torres, Von V L; Bi, Wenzi; Mandela, Eric; Cao, Jianming; Li, Jiahui; Lithgow, Trevor; Zhou, Tieli.
Afiliación
  • Chen L; Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, PR China.
  • Wilksch JJ; Infection and Immunity Program, Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, Victoria, Australia.
  • Liu H; Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University Hangzhou, China; Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, PR China.
  • Zhang X; Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, PR China.
  • Torres VVL; Infection and Immunity Program, Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, Victoria, Australia.
  • Bi W; School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, Zhejiang Province, PR China.
  • Mandela E; Infection and Immunity Program, Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, Victoria, Australia.
  • Cao J; School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, Zhejiang Province, PR China.
  • Li J; Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, PR China.
  • Lithgow T; Infection and Immunity Program, Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, Victoria, Australia.
  • Zhou T; Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, PR China.
J Med Microbiol ; 69(3): 402-413, 2020 Mar.
Article en En | MEDLINE | ID: mdl-32223838
Introduction. Autoinducer-2 (AI-2) quorum sensing is a bacterial communication system that responds to cell density. The system requires luxS activity to produce AI-2, which can regulate gene expression and processes such as biofilm formation.Aim. To investigate the role of luxS in biofilm formation and gene expression in the nosocomial pathogen Klebsiella pneumoniae.Methodology. A ΔluxS gene deletion was made in K. pneumoniae KP563, an extensively drug-resistant isolate. AI-2 production was assessed in wild-type and ΔluxS strains grown in media supplemented with different carbohydrates. Potential roles of luxS in biofilm formation were investigated using a microtiter plate biofilm assay and scanning electron microscopy. Quantitative RT-PCR evaluated the expression of lipopolysaccharide (wzm and wbbM), polysaccharide (pgaA), and type 3 fimbriae (mrkA) synthesis genes in wild-type and ΔluxS mutant biofilm extracts.Results. AI-2 production was dependent on the presence of luxS. AI-2 accumulation was highest during early stationary phase in media supplemented with glucose, sucrose or glycerol. Changes in biofilm architecture were observed in the ΔluxS mutant, with less surface coverage and reduced macrocolony formation; however, no differences in biofilm formation between the wild-type and ΔluxS mutant using a microtiter plate assay were observed. In ΔluxS mutant biofilm extracts, the expression of wzm was down-regulated, and the expression of pgaA, which encodes a porin for poly-ß-1,6-N-acetyl-d-glucosamine (PNAG) polysaccharide secretion, was upregulated.Conclusion. Relationships among AI-2-mediated quorum sensing, biofilm formation and gene expression of outer-membrane components were identified in K. pneumoniae. These inter-connected processes could be important for bacterial group behaviour and persistence.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Liasas de Carbono-Azufre / Proteínas Bacterianas / Infecciones por Klebsiella / Biopelículas / Percepción de Quorum / Klebsiella pneumoniae Límite: Humans Idioma: En Revista: J Med Microbiol Año: 2020 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Liasas de Carbono-Azufre / Proteínas Bacterianas / Infecciones por Klebsiella / Biopelículas / Percepción de Quorum / Klebsiella pneumoniae Límite: Humans Idioma: En Revista: J Med Microbiol Año: 2020 Tipo del documento: Article Pais de publicación: Reino Unido