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Effect of lithium carbonate on autophagy and proliferative activity of isolated hepatocytes.
Dossymbekova, Raushan; Bgatova, Nataliya; Tungushbayeva, Zina; Sharipov, Kamalidin; Taneyeva, Gulzhan; Kydyrbaeva, Asem; Solovieva, Anastasiya.
Afiliación
  • Dossymbekova R; Department of Biology, Institute of Natural Sciences and Geography, Abai Kazakh National Pedagogical University, Abai Kazakh National Pedagogical University, Kazakhstan. Electronic address: dos.raushan@mail.ru.
  • Bgatova N; Research Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Siberian Branch of Russian Academy of Sciences, Russia.
  • Tungushbayeva Z; Department of Biology, Institute of Natural Sciences and Geography, Abai Kazakh National Pedagogical University, Abai Kazakh National Pedagogical University, Kazakhstan.
  • Sharipov K; Department of Biological Chemistry, Kazakh National Medical University named after S.D. Asfendiyarov, S.D. Asfendiyarov Kazakh National Medical University, Kazakhstan.
  • Taneyeva G; Department of Molecular Biology and Medical Genetics, Kazakh National Medical University named after S.D. Asfendiyarov, S.D. Asfendiyarov Kazakh National Medical University, Kazakhstan.
  • Kydyrbaeva A; Department of Molecular Biology and Medical Genetics, Kazakh National Medical University named after S.D. Asfendiyarov, S.D. Asfendiyarov Kazakh National Medical University, Kazakhstan.
  • Solovieva A; Laboratory of Pharmacological Active Compounds Research Institute of Clinical and Experimental Lymphology, Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Siberian Branch of Russian Academy of Sciences, Russia.
Biochem Biophys Res Commun ; 528(2): 343-346, 2020 07 23.
Article en En | MEDLINE | ID: mdl-32209260
The hepatocytes were cultivated in the presence of lithium carbonate (LC) for drugs testing or possible source for transplantation in the treatment of hereditary or terminal liver diseases. The LC, as an inducer of autophagy, is a promising drug for maintaining cell homeostasis and has a significant effect on the ultrastructural organization of hepatocyte cells. Within current investigation, new mechanisms of the biological effects of lithium and the ultrastructural analysis of the primary culture of hepatocytes were studied via flow cytofluorometry, light, and electron microscopy methods. Obtained results demonstrate the absence of the toxic effect of 5 mM of LC on the primary hepatocyte culture. In addition, LC does not block the cell cycle at the G0/G1 stage after 24 h of hepatocyte cultivation and promotes the preservation of their viability by 48 h of the experiment. Moreover, LC does not stimulate hepatocyte apoptosis, induces autophagy and the preserves the proliferative activity of hepatocytes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Autofagia / Separación Celular / Carbonato de Litio / Hepatocitos Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Autofagia / Separación Celular / Carbonato de Litio / Hepatocitos Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos