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Production of volatile compounds by yeasts using hydrolysed grape seed oil obtained by immobilized lipases in continuous packed-bed reactors.
Castiglioni, Gabriele Zanota; Bettio, Giulia; Matte, Carla Roberta; Jacques, Rosangela Assis; Dos Santos Polidoro, Allan; Rosa, Carlos Augusto; Ayub, Marco Antônio Záchia.
Afiliación
  • Castiglioni GZ; Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande Do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil.
  • Bettio G; Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande Do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil.
  • Matte CR; Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande Do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil.
  • Jacques RA; Chemistry Institute, Federal University of Rio Grande Do Sul, Porto Alregre, RS, Brazil.
  • Dos Santos Polidoro A; Chemistry Institute, Federal University of Rio Grande Do Sul, Porto Alregre, RS, Brazil.
  • Rosa CA; Department of Microbiology, ICB, C.P. 486, State University of Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
  • Ayub MAZ; Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande Do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil. mazayub@ufrgs.br.
Bioprocess Biosyst Eng ; 43(8): 1391-1402, 2020 Aug.
Article en En | MEDLINE | ID: mdl-32206907
Lipases CAL-B, TLL, and RML were used in the synthesis of free fatty acids of grape seed oil as heterogeneous substrate. The best enzyme was used to optimize the reaction variables temperature, enzyme content, and molar ratio of water:oil in batch reactions using experimental planning. The ideal conditions to produce free fatty acids using pure RML were 45 °C, 12:1 substrate molar ratio, and 15% enzyme, resulting in 66% of oil hydrolysis and a productivity of 0.54 mol L-1 min-1 in 4 h of reaction at 180 rpm. Repeated batches of reaction were performed testing the operational stability of RML, results showing that this enzyme could be used for at least 20 cycles keeping more than 80% of its initial activity, suggesting its potential use in industrial processes. The synthesis of free fatty acids was then evaluated in continuous reactions using packed-bed reactor (PBR). The highest productivity in the continuous process was 6.85 mol L-1 min-1, using only RML, showing an operational stability higher than 80% of its initial conversion capacity after 11 days of operation, at a flow rate of 0.13 mL min-1 at 45 °C. We evaluated the use of this hydrolyzed oil as substrate for lactone bioproduction using Galactomyces geotrichum UFMG-CM-Y3276, G. geotrichum UFMG-CM-Y3558, and Geotrichum klebahnii UFMG-CM-Y3014 screened for their oil-hydrolysis ability. Volatile compounds were qualitatively identified in GC-MS as γ-octalactone and γ-nonalactone.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semillas / Aceites de Plantas / Vitis / Enzimas Inmovilizadas / Compuestos Orgánicos Volátiles / Geotrichum / Lipasa Tipo de estudio: Prognostic_studies Idioma: En Revista: Bioprocess Biosyst Eng Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semillas / Aceites de Plantas / Vitis / Enzimas Inmovilizadas / Compuestos Orgánicos Volátiles / Geotrichum / Lipasa Tipo de estudio: Prognostic_studies Idioma: En Revista: Bioprocess Biosyst Eng Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania