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An Experimental Study Comparing the Expansion of Peripheral Blood Natural Killer (NK) Cells Cultured with Artificial Antigen-Presenting Cells, in the Presence or Absence of Bone Marrow Mesenchymal Stem Cells (MSCs).
Pedroso, Jaiza Frias; de Souza Valim, Vanessa; Pezzi, Annelise; Furlan, Juliana Monteiro; Lenhart, Gabriele; Sehn, Filipe; Zambonato, Bruna; Gonçalves, Alice Dahmer; Wilke, Ianaê; Amorin, Bruna; da Silva, Maria Aperecida; Pedrazzani, Fabiane Spagnol; da Rocha Silla, Lucia Mariano.
Afiliación
  • Pedroso JF; Cellular Technology and Therapy Center, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos, 2350, Porto Alegre, RS, CEP 90035-903, Brazil.
  • de Souza Valim V; Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil.
  • Pezzi A; Cellular Technology and Therapy Center, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos, 2350, Porto Alegre, RS, CEP 90035-903, Brazil.
  • Furlan JM; Cellular Technology and Therapy Center, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos, 2350, Porto Alegre, RS, CEP 90035-903, Brazil.
  • Lenhart G; Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil.
  • Sehn F; Cellular Technology and Therapy Center, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos, 2350, Porto Alegre, RS, CEP 90035-903, Brazil.
  • Zambonato B; Cellular Technology and Therapy Center, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos, 2350, Porto Alegre, RS, CEP 90035-903, Brazil.
  • Gonçalves AD; Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil.
  • Wilke I; Cellular Technology and Therapy Center, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos, 2350, Porto Alegre, RS, CEP 90035-903, Brazil.
  • Amorin B; Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil.
  • da Silva MA; Cellular Technology and Therapy Center, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos, 2350, Porto Alegre, RS, CEP 90035-903, Brazil.
  • Pedrazzani FS; Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil.
  • da Rocha Silla LM; Cellular Technology and Therapy Center, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos, 2350, Porto Alegre, RS, CEP 90035-903, Brazil.
Mol Biotechnol ; 62(5): 306-315, 2020 May.
Article en En | MEDLINE | ID: mdl-32193710
NK cells have been seen as potential agents in adoptive immunotherapy for cancer. The main challenge for the success of this approach is to obtain a great quantity of activated NK cells for adoptive transfer. The present study had aimed to evaluate the effect of a feeder layer of irradiated MSCs in the in vitro expansion of NK cells. MSCs were obtained from the bone marrow (BM) cells remaining in the bag and filter used in the transplantation of hematopoietic stem cells. NK cells were obtained from peripheral blood (PB) of healthy volunteers. NK expansion and activation were stimulated by culture with artificial antigen-presenting cells (aAPCs) and IL-2, in the presence or absence of BM-MSCs. NK cell proliferation, phenotypic expression and cytotoxic activity were evaluated. Both culture conditions showed high NK purity with predominance of NK CD56brightCD16+ subset post expansion. However, cultures without the presence of MSCs showed higher NK proliferation, expression of activation markers (CD16 and NKG2D) and related cytotoxic activity. In this experimental study, the presence of a feeder layer of irradiated BM-MSCs interfered negatively in the expansion of PB-NKs, limiting their growth and activation. Further investigation is needed to understand the mechanisms of NK-MSC interaction and its implications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucocitos Mononucleares / Células Asesinas Naturales / Células Madre Mesenquimatosas / Células Presentadoras de Antígenos Límite: Humans Idioma: En Revista: Mol Biotechnol Asunto de la revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucocitos Mononucleares / Células Asesinas Naturales / Células Madre Mesenquimatosas / Células Presentadoras de Antígenos Límite: Humans Idioma: En Revista: Mol Biotechnol Asunto de la revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza