Community dynamics of As(V)-reducing and As(III)-oxidizing genes during a wet-dry cycle in paddy soil amended with organic matter, gypsum, or iron oxide.
J Hazard Mater
; 393: 122485, 2020 07 05.
Article
en En
| MEDLINE
| ID: mdl-32193132
Microbe-mediated redox transformations regulate arsenic mobility in paddy soil. However, the community dynamics of the related genes, which might be affected by soil ameliorants, have not been systematically investigated during a wet-dry cycle. This study incubated arsenic-contaminated paddy soil amended with organic matter (OM), gypsum, or hematite in microcosms under alternate watering conditions. Added gypsum and hematite reduced arsenic mobility in the soil by 8-60% during the wet and dry periods. However, added OM increased arsenic mobility by 70-130% during the first 4 weeks (not the last 4 weeks) of submergence and the dry period. The results of quantitative real-time polymerase chain reaction (qPCR) depended heavily on the primers used, so the contribution of relevant genes to arsenic transformation cannot be compared using only the gene abundance assessed by qPCR. However, correlation analyses showed that the abundance and community members of the arrA gene, which mediates dissimilatory As(V) reduction [i.e., As(V) respiration], were related to soil arsenic concentrations. This was not the case for the arsC gene, which mediates cytoplasmic As(V) reduction, or the aioA gene, which mediates As(III) oxidation. These suggest that the dissimilatory pathway was mainly responsible for arsenic reduction and release in the soil studied.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Arsénico
/
Microbiología del Suelo
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Contaminantes del Suelo
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Sulfato de Calcio
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Compuestos Férricos
/
Genes Bacterianos
Idioma:
En
Revista:
J Hazard Mater
Asunto de la revista:
SAUDE AMBIENTAL
Año:
2020
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Países Bajos