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Homeodomain-interacting protein kinase (Hipk) plays roles in nervous system and muscle structure and function.
Wang, Simon J H; Sinclair, Donald A R; Kim, Hae-Yoon; Kinsey, Stephen D; Yoo, Byoungjoo; Shih, Claire R Y; Wong, Kenneth K L; Krieger, Charles; Harden, Nicholas; Verheyen, Esther M.
Afiliación
  • Wang SJH; Centre for Cell Biology, Development and Disease, Simon Fraser University, Burnaby, Canada.
  • Sinclair DAR; Department of Biomedical Physiology and Kinesiology, Simon Fraser University, Burnaby, Canada.
  • Kim HY; Centre for Cell Biology, Development and Disease, Simon Fraser University, Burnaby, Canada.
  • Kinsey SD; Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, Canada.
  • Yoo B; Centre for Cell Biology, Development and Disease, Simon Fraser University, Burnaby, Canada.
  • Shih CRY; Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, Canada.
  • Wong KKL; Centre for Cell Biology, Development and Disease, Simon Fraser University, Burnaby, Canada.
  • Krieger C; Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, Canada.
  • Harden N; Centre for Cell Biology, Development and Disease, Simon Fraser University, Burnaby, Canada.
  • Verheyen EM; Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, Canada.
PLoS One ; 15(3): e0221006, 2020.
Article en En | MEDLINE | ID: mdl-32187190
Homeodomain-interacting protein kinases (Hipks) have been previously associated with cell proliferation and cancer, however, their effects in the nervous system are less well understood. We have used Drosophila melanogaster to evaluate the effects of altered Hipk expression on the nervous system and muscle. Using genetic manipulation of Hipk expression we demonstrate that knockdown and over-expression of Hipk produces early adult lethality, possibly due to the effects on the nervous system and muscle involvement. We find that optimal levels of Hipk are critical for the function of dopaminergic neurons and glial cells in the nervous system, as well as muscle. Furthermore, manipulation of Hipk affects the structure of the larval neuromuscular junction (NMJ) by promoting its growth. Hipk regulates the phosphorylation of the synapse-associated cytoskeletal protein Hu-li tai shao (Hts; adducin in mammals) and modulates the expression of two important protein kinases, Calcium-calmodulin protein kinase II (CaMKII) and Partitioning-defective 1 (PAR-1), all of which may alter neuromuscular structure/function and influence lethality. Hipk also modifies the levels of an important nuclear protein, TBPH, the fly orthologue of TAR DNA-binding protein 43 (TDP-43), which may have relevance for understanding motor neuron diseases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Quinasas / Proteínas de Drosophila / Drosophila melanogaster / Músculos / Sistema Nervioso Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2020 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Quinasas / Proteínas de Drosophila / Drosophila melanogaster / Músculos / Sistema Nervioso Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2020 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos