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Sensory-Directed Genetic and Biochemical Characterization of Volatile Terpene Production in Kiwifruit.
Zeng, Yunliu; Wang, Mindy Y; Hunter, Denise C; Matich, Adam J; McAtee, Peter A; Knäbel, Mareike; Hamiaux, Cyril; Popowski, Elizabeth A; Jaeger, Sara R; Nieuwenhuizen, Niels J; Yauk, Yar-Khing; Atkinson, Ross G.
Afiliación
  • Zeng Y; Key Laboratory of Horticultural Plant Biology, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, P.R. China.
  • Wang MY; The New Zealand Institute for Plant and Food Research Ltd. (PFR), Auckland 1142, New Zealand.
  • Hunter DC; The New Zealand Institute for Plant and Food Research Ltd. (PFR), Auckland 1142, New Zealand.
  • Matich AJ; The New Zealand Institute for Plant and Food Research Ltd. (PFR), Auckland 1142, New Zealand.
  • McAtee PA; PFR, Palmerston North 4442, New Zealand.
  • Knäbel M; The New Zealand Institute for Plant and Food Research Ltd. (PFR), Auckland 1142, New Zealand.
  • Hamiaux C; PFR, Palmerston North 4442, New Zealand.
  • Popowski EA; The New Zealand Institute for Plant and Food Research Ltd. (PFR), Auckland 1142, New Zealand.
  • Jaeger SR; PFR, Te Puke 3182, New Zealand.
  • Nieuwenhuizen NJ; The New Zealand Institute for Plant and Food Research Ltd. (PFR), Auckland 1142, New Zealand.
  • Yauk YK; The New Zealand Institute for Plant and Food Research Ltd. (PFR), Auckland 1142, New Zealand.
  • Atkinson RG; The New Zealand Institute for Plant and Food Research Ltd. (PFR), Auckland 1142, New Zealand.
Plant Physiol ; 183(1): 51-66, 2020 05.
Article en En | MEDLINE | ID: mdl-32184346
Terpene volatiles are found in many important fruit crops, but their relationship to flavor is poorly understood. Here, we demonstrate using sensory descriptive and discriminant analysis that 1,8-cineole contributes a key floral/eucalyptus note to the aroma of ripe 'Hort16A' kiwifruit (Actinidia chinensis). Two quantitative trait loci (QTLs) for 1,8-cineole production were identified on linkage groups 27 and 29a in a segregating A. chinensis population, with the QTL on LG29a colocating with a complex cluster of putative terpene synthase (TPS)-encoding genes. Transient expression in Nicotiana benthamiana and analysis of recombinant proteins expressed in Escherichia coli showed four genes in the cluster (AcTPS1a-AcTPS1d) encoded functional TPS enzymes, which produced predominantly sabinene, 1,8-cineole, geraniol, and springene, respectively. The terpene profile produced by AcTPS1b closely resembled the terpenes detected in red-fleshed A chinensis AcTPS1b expression correlated with 1,8-cineole content in developing/ripening fruit and also showed a positive correlation with 1,8-cineole content in the mapping population, indicating the basis for segregation is an expression QTL. Transient overexpression of AcTPS1b in Actinidia eriantha fruit confirmed this gene produced 1,8-cineole in Actinidia Structure-function analysis showed AcTPS1a and AcTPS1b are natural variants at key TPS catalytic site residues previously shown to change enzyme specificity in vitro. Together, our results indicate that AcTPS1b is a key gene for production of the signature flavor terpene 1,8-cineole in ripe kiwifruit. Using a sensory-directed strategy for compound identification provides a rational approach for applying marker-aided selection to improving flavor in kiwifruit as well as other fruits.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Terpenos / Transferasas Alquil y Aril / Actinidia / Frutas Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Physiol Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Terpenos / Transferasas Alquil y Aril / Actinidia / Frutas Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Physiol Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos