Protein Engineering of a Pyridoxal-5'-Phosphate-Dependent l-Aspartate-&#945;-Decarboxylase from <i>Tribolium castaneum</i> for ß-Alanine Production.

Yu, Xin-Jun; Huang, Chang-Yi; Xu, Xiao-Dan; Chen, Hong; Liang, Miao-Jie; Xu, Zhe-Xian; Xu, Hui-Xia; Wang, Zhao

Protein Engineering of a Pyridoxal-5'-Phosphate-Dependent l-Aspartate-α-Decarboxylase from Tribolium castaneum for ß-Alanine Production.

Yu, Xin-Jun; Huang, Chang-Yi; Xu, Xiao-Dan; Chen, Hong; Liang, Miao-Jie; Xu, Zhe-Xian; Xu, Hui-Xia; Wang, Zhao.

Afiliación

Yu XJ; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No.18, Chaowang Road, Hangzhou 310014, China.
Huang CY; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No.18, Chaowang Road, Hangzhou 310014, China.
Xu XD; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No.18, Chaowang Road, Hangzhou 310014, China.
Chen H; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No.18, Chaowang Road, Hangzhou 310014, China.
Liang MJ; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No.18, Chaowang Road, Hangzhou 310014, China.
Xu ZX; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No.18, Chaowang Road, Hangzhou 310014, China.
Xu HX; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No.18, Chaowang Road, Hangzhou 310014, China.
Wang Z; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No.18, Chaowang Road, Hangzhou 310014, China.

Molecules ; 25(6)2020 Mar 12.

Article en En | MEDLINE | ID: mdl-32178239

Asunto(s)

Glutamato Descarboxilasa/genética; Ingeniería de Proteínas/métodos; Fosfato de Piridoxal/metabolismo; beta-Alanina/biosíntesis; Animales; Escherichia coli/genética; Glutamato Descarboxilasa/química; Cinética; Fosfato de Piridoxal/química; Tribolium/enzimología; Tribolium/genética; beta-Alanina/química

Palabras clave

characterization; directed evolution; l-aspartate-α-decarboxylase; pyridoxal-5'-phosphate (PLP); whole-cell bioconversion; ß-alanine

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfato de Piridoxal / Ingeniería de Proteínas / Beta-Alanina / Glutamato Descarboxilasa Límite: Animals Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza

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