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Antitumor activity of nervosine VII, and the crosstalk between apoptosis and autophagy in HCT116 human colorectal cancer cells.
Huang, Shuai; Zhao, Shuang-Mei; Shan, Lian-Hai; Zhou, Xian-Li.
Afiliación
  • Huang S; School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China. Electronic address: shuaih@swjtu.edu.cn.
  • Zhao SM; School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
  • Shan LH; School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
  • Zhou XL; School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China. Electronic address: zhouxl@swjtu.edu.cn.
Chin J Nat Med ; 18(2): 81-89, 2020 Feb.
Article en En | MEDLINE | ID: mdl-32172951
Nervosine VII is one of the known saturated pyrrolizidine alkaloids isolated from the plant of Liparis nervosa. This is first study to investigate the antitumor activity of nervosine VII in vitro, and the results indicated that nervosine VII induced autophagy and apoptosis in HCT116 human colorectal cancer cells. Mechanistic studies showed that nervosine VII-induced apoptosis was associated with the intrinsic pathway by the activation of caspase-9, -3 and -7. Autophagy induced by nervosine VII was characteristic with the regulation of autophagic markers including the increase of LC3-II and beclin 1 proteins, and the decrease of p62 protein. Nervosine VII simultaneously induced autophagy and apoptosis by activated MAPKs signaling pathway including JNK, ERK1/2 and p38, suppressing the p53 signaling pathway.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alcaloides de Pirrolicidina / Autofagia / Extractos Vegetales / Apoptosis / Antineoplásicos Límite: Humans Idioma: En Revista: Chin J Nat Med Año: 2020 Tipo del documento: Article Pais de publicación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alcaloides de Pirrolicidina / Autofagia / Extractos Vegetales / Apoptosis / Antineoplásicos Límite: Humans Idioma: En Revista: Chin J Nat Med Año: 2020 Tipo del documento: Article Pais de publicación: China