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Excitation dynamics and relaxation in the major antenna of a marine green alga Bryopsis corticulans.
Li, Dan-Hong; Wang, Wenda; Zhou, Cuicui; Zhang, Yan; Wang, Peng; Shen, Jian-Ren; Kuang, Tingyun; Zhang, Jian-Ping.
Afiliación
  • Li DH; Department of Chemistry, Renmin University of China, Beijing 100872, China. Electronic address: lidh01@ruc.edu.cn.
  • Wang W; Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 100093 Beijing, China. Electronic address: wdwang@ibcas.ac.cn.
  • Zhou C; Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 100093 Beijing, China. Electronic address: zhoucc@ibcas.ac.cn.
  • Zhang Y; Department of Chemistry, Renmin University of China, Beijing 100872, China. Electronic address: yerzhang@ruc.edu.cn.
  • Wang P; Department of Chemistry, Renmin University of China, Beijing 100872, China. Electronic address: wpeng_chem@ruc.edu.cn.
  • Shen JR; Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 100093 Beijing, China. Electronic address: shen@cc.okayama-u.ac.jp.
  • Kuang T; Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 100093 Beijing, China. Electronic address: kuangty@ibcas.ac.cn.
  • Zhang JP; Department of Chemistry, Renmin University of China, Beijing 100872, China. Electronic address: jpzhang@ruc.edu.cn.
Biochim Biophys Acta Bioenerg ; 1861(5-6): 148186, 2020 06 01.
Article en En | MEDLINE | ID: mdl-32171793
The light-harvesting complexes II (LHCIIs) of spinach and Bryopsis corticulans as a green alga are similar in structure, but differ in carotenoid (Car) and chlorophyll (Chl) compositions. Carbonyl Cars siphonein (Spn) and siphonaxanthin (Spx) bind to B. corticulans LHCII likely in the sites as a pair of lutein (Lut) molecules bind to spinach LHCII in the central domain. To understand the light-harvesting and photoprotective properties of the algal LHCII, we compared its excitation dynamics and relaxation to those of spinach LHCII been well documented. It was found that B. corticulans LHCII exhibited a substantially longer chlorophyll (Chl) fluorescence lifetime (4.9 ns vs 4.1 ns) and a 60% increase of the fluorescence quantum yield. Photoexcitation populated 3Car* equally between Spn and Spx in B. corticulans LHCII, whereas predominantly at Lut620 in spinach LHCII. These results prove the functional differences of the LHCIIs with different Car pairs and Chl a/b ratios: B. corticulans LHCII shows the enhanced blue-green light absorption, the alleviated quenching of 1Chl*, and the dual sites of quenching 3Chl*, which may facilitate its light-harvesting and photoprotection functions. Moreover, for both types of LHCIIs, the triplet excitation profiles revealed the involvement of extra 3Car* formation mechanisms besides the conventional Chl-to-Car triplet transfer, which are discussed in relation to the ultrafast processes of 1Chl* quenching. Our experimental findings will be helpful in deepening the understanding of the light harvesting and photoprotection functions of B. corticulans living in the intertidal zone with dramatically changing light condition.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Agua de Mar / Complejos de Proteína Captadores de Luz / Chlorophyta Idioma: En Revista: Biochim Biophys Acta Bioenerg Año: 2020 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Agua de Mar / Complejos de Proteína Captadores de Luz / Chlorophyta Idioma: En Revista: Biochim Biophys Acta Bioenerg Año: 2020 Tipo del documento: Article Pais de publicación: Países Bajos