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Stimulation of L-type calcium channels increases tyrosine hydroxylase and dopamine in ventral midbrain cells induced from somatic cells.
Jefri, Malvin; Bell, Scott; Peng, Huashan; Hettige, Nuwan; Maussion, Gilles; Soubannier, Vincent; Wu, Hanrong; Silveira, Heika; Theroux, Jean-Francois; Moquin, Luc; Zhang, Xin; Aouabed, Zahia; Krishnan, Jeyashree; O'Leary, Liam A; Antonyan, Lilit; Zhang, Ying; McCarty, Vincent; Mechawar, Naguib; Gratton, Alain; Schuppert, Andreas; Durcan, Thomas M; Fon, Edward A; Ernst, Carl.
Afiliación
  • Jefri M; Psychiatric Genetics Group, McGill University, Montreal, Quebec, Canada.
  • Bell S; Department of Psychiatry, McGill University and Douglas Hospital Research Institute, Montreal, Quebec, Canada.
  • Peng H; Psychiatric Genetics Group, McGill University, Montreal, Quebec, Canada.
  • Hettige N; Department of Psychiatry, McGill University and Douglas Hospital Research Institute, Montreal, Quebec, Canada.
  • Maussion G; Psychiatric Genetics Group, McGill University, Montreal, Quebec, Canada.
  • Soubannier V; Department of Psychiatry, McGill University and Douglas Hospital Research Institute, Montreal, Quebec, Canada.
  • Wu H; Psychiatric Genetics Group, McGill University, Montreal, Quebec, Canada.
  • Silveira H; Department of Psychiatry, McGill University and Douglas Hospital Research Institute, Montreal, Quebec, Canada.
  • Theroux JF; Department of Neurology and Neurosurgery, Montreal Neurological Institute, Montreal, Quebec, Canada.
  • Moquin L; Department of Neurology and Neurosurgery, Montreal Neurological Institute, Montreal, Quebec, Canada.
  • Zhang X; Psychiatric Genetics Group, McGill University, Montreal, Quebec, Canada.
  • Aouabed Z; Department of Psychiatry, McGill University and Douglas Hospital Research Institute, Montreal, Quebec, Canada.
  • Krishnan J; Psychiatric Genetics Group, McGill University, Montreal, Quebec, Canada.
  • O'Leary LA; Department of Psychiatry, McGill University and Douglas Hospital Research Institute, Montreal, Quebec, Canada.
  • Antonyan L; Psychiatric Genetics Group, McGill University, Montreal, Quebec, Canada.
  • Zhang Y; Department of Psychiatry, McGill University and Douglas Hospital Research Institute, Montreal, Quebec, Canada.
  • McCarty V; Department of Psychiatry, McGill University and Douglas Hospital Research Institute, Montreal, Quebec, Canada.
  • Mechawar N; Psychiatric Genetics Group, McGill University, Montreal, Quebec, Canada.
  • Gratton A; Department of Psychiatry, McGill University and Douglas Hospital Research Institute, Montreal, Quebec, Canada.
  • Schuppert A; Psychiatric Genetics Group, McGill University, Montreal, Quebec, Canada.
  • Durcan TM; Department of Psychiatry, McGill University and Douglas Hospital Research Institute, Montreal, Quebec, Canada.
  • Fon EA; Institute for Computational Biomedicine, Aachen University, Aachen, Germany.
  • Ernst C; Department of Psychiatry, McGill University and Douglas Hospital Research Institute, Montreal, Quebec, Canada.
Stem Cells Transl Med ; 9(6): 697-712, 2020 Jun.
Article en En | MEDLINE | ID: mdl-32154672
Making high-quality dopamine (DA)-producing cells for basic biological or small molecule screening studies is critical for the development of novel therapeutics for disorders of the ventral midbrain. Currently, many ventral midbrain assays have low signal-to-noise ratio due to low levels of cellular DA and the rate-limiting enzyme of DA synthesis, tyrosine hydroxylase (TH), hampering discovery efforts. Using intensively characterized ventral midbrain cells derived from human skin, which demonstrate calcium pacemaking activity and classical electrophysiological properties, we show that an L-type calcium agonist can significantly increase TH protein levels and DA content and release. Live calcium imaging suggests that it is the immediate influx of calcium occurring simultaneously in all cells that drives this effect. Genome-wide expression profiling suggests that L-type calcium channel stimulation has a significant effect on specific genes related to DA synthesis and affects expression of L-type calcium receptor subunits from the CACNA1 and CACNA2D families. Together, our findings provide an advance in the ability to increase DA and TH levels to improve the accuracy of disease modeling and small molecule screening for disorders of the ventral midbrain, including Parkinson's disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tirosina 3-Monooxigenasa / Mesencéfalo / Dopamina / Canales de Calcio Tipo L Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Stem Cells Transl Med Año: 2020 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tirosina 3-Monooxigenasa / Mesencéfalo / Dopamina / Canales de Calcio Tipo L Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Stem Cells Transl Med Año: 2020 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido