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A bifunctional O-antigen polymerase structure reveals a new glycosyltransferase family.
Clarke, Bradley R; Ovchinnikova, Olga G; Sweeney, Ryan P; Kamski-Hennekam, Evelyn R; Gitalis, Russel; Mallette, Evan; Kelly, Steven D; Lowary, Todd L; Kimber, Matthew S; Whitfield, Chris.
Afiliación
  • Clarke BR; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.
  • Ovchinnikova OG; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.
  • Sweeney RP; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Kamski-Hennekam ER; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.
  • Gitalis R; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.
  • Mallette E; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.
  • Kelly SD; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.
  • Lowary TL; Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
  • Kimber MS; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada. mkimber@uoguelph.ca.
  • Whitfield C; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada. cwhitfie@uoguelph.ca.
Nat Chem Biol ; 16(4): 450-457, 2020 04.
Article en En | MEDLINE | ID: mdl-32152541
Lipopolysaccharide O-antigen is an attractive candidate for immunotherapeutic strategies targeting antibiotic-resistant Klebsiella pneumoniae. Several K. pneumoniae O-serotypes are based on a shared O2a-antigen backbone repeating unit: (→ 3)-α-Galp-(1 → 3)-ß-Galf-(1 →). O2a antigen is synthesized on undecaprenol diphosphate in a pathway involving the O2a polymerase, WbbM, before its export by an ATP-binding cassette transporter. This dual domain polymerase possesses a C-terminal galactopyranosyltransferase resembling known GT8 family enzymes, and an N-terminal DUF4422 domain identified here as a galactofuranosyltransferase defining a previously unrecognized family (GT111). Functional assignment of DUF4422 explains how galactofuranose is incorporated into various polysaccharides of importance in vaccine production and the food industry. In the 2.1-Å resolution structure, three WbbM protomers associate to form a flattened triangular prism connected to a central stalk that orients the active sites toward the membrane. The biochemical, structural and topological properties of WbbM offer broader insight into the mechanisms of assembly of bacterial cell-surface glycans.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicosiltransferasas / Antígenos O Idioma: En Revista: Nat Chem Biol Asunto de la revista: BIOLOGIA / QUIMICA Año: 2020 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicosiltransferasas / Antígenos O Idioma: En Revista: Nat Chem Biol Asunto de la revista: BIOLOGIA / QUIMICA Año: 2020 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos