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Cost analysis based on bioreactor cultivation conditions: Production of a soluble recombinant protein using Escherichia coli BL21(DE3).
Cardoso, Valdemir M; Campani, Gilson; Santos, Maurício P; Silva, Gabriel G; Pires, Manuella C; Gonçalves, Viviane M; de C Giordano, Roberto; Sargo, Cíntia R; Horta, Antônio C L; Zangirolami, Teresa C.
Afiliación
  • Cardoso VM; Graduate Program of Chemical Engineering (PPGEQ), Federal University of São Carlos (UFSCar), Rodovia Washington Luís, km 235, 13565-905, São Carlos, SP, Brazil.
  • Campani G; Graduate Program of Chemical Engineering (PPGEQ), Federal University of São Carlos (UFSCar), Rodovia Washington Luís, km 235, 13565-905, São Carlos, SP, Brazil.
  • Santos MP; Department of Engineering, Federal University of Lavras, 37200-000, Lavras, MG, Brazil.
  • Silva GG; Graduate Program of Chemical Engineering (PPGEQ), Federal University of São Carlos (UFSCar), Rodovia Washington Luís, km 235, 13565-905, São Carlos, SP, Brazil.
  • Pires MC; Graduate Program of Chemical Engineering (PPGEQ), Federal University of São Carlos (UFSCar), Rodovia Washington Luís, km 235, 13565-905, São Carlos, SP, Brazil.
  • Gonçalves VM; Laboratory of Vaccine Development, Butantan Institute, Av. Vital Brasil 1500, 05508-900, São Paulo, SP, Brazil.
  • de C Giordano R; Laboratory of Vaccine Development, Butantan Institute, Av. Vital Brasil 1500, 05508-900, São Paulo, SP, Brazil.
  • Sargo CR; Graduate Program of Chemical Engineering (PPGEQ), Federal University of São Carlos (UFSCar), Rodovia Washington Luís, km 235, 13565-905, São Carlos, SP, Brazil.
  • Horta ACL; Graduate Program of Chemical Engineering (PPGEQ), Federal University of São Carlos (UFSCar), Rodovia Washington Luís, km 235, 13565-905, São Carlos, SP, Brazil.
  • Zangirolami TC; Brazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM), 13083-970, Campinas, SP, Brazil.
Biotechnol Rep (Amst) ; 26: e00441, 2020 Jun.
Article en En | MEDLINE | ID: mdl-32140446
The impact of cultivation strategy on the cost of recombinant protein production is crucial for defining cost-effective bioreactor operation conditions. This paper presents a methodology to estimate and compare cost impacts related to utilities as well as medium composition, using simple design equations and accessible data. Data from batch bioreactor cultures were used as case study involving the production of pneumococcal surface protein A, a soluble recombinant protein, employing E. coli BL21(DE3). Cultivation strategies and corresponding process costs covered a wide range of operational conditions, including different media, inducers, and temperatures. The core expenses were related to the medium and cooling. When the price of peptone was above the threshold value of US$ 30/kg, defined medium became the best choice. IPTG and temperatures around 32 °C led to shorter cultures and lower PspA4Pro production costs. The procedure offers a simple, accessible theoretical tool to identify cost-effective production strategies using bioreactors.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Health_economic_evaluation Idioma: En Revista: Biotechnol Rep (Amst) Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Health_economic_evaluation Idioma: En Revista: Biotechnol Rep (Amst) Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos