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Methods for Cryosectioning and Mass Spectrometry Imaging of Whole-Body Zebrafish.
Stutts, Whitney L; Knuth, Megan M; Ekelöf, Måns; Mahapatra, Debabrata; Kullman, Seth W; Muddiman, David C.
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  • Stutts WL; Molecular Education, Technology, and Research Innovation Center, North Carolina State University, Raleigh, North Carolina 27695, United States.
  • Knuth MM; Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina 27695, United States.
  • Ekelöf M; FTMS Laboratory for Human Health Research and Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States.
  • Mahapatra D; Comparative Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27695, United States.
  • Kullman SW; Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina 27695, United States.
  • Muddiman DC; Comparative Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27695, United States.
J Am Soc Mass Spectrom ; 31(4): 768-772, 2020 Apr 01.
Article en En | MEDLINE | ID: mdl-32129621
The zebrafish (Danio rerio) is an ideal model for whole animal studies of lipid metabolism and lipid-related disease. In this work, infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) was applied for direct visualization of lipid and metabolite distributions across various organs in whole-body zebrafish tissue sections. Detailed methods for overcoming the challenges of cryosectioning adult male zebrafish for MSI and complementary histological imaging are described. Representative two-dimensional ion maps demonstrated organ specific localization of lipid analytes allowing for visualization of areas of interest including the brain, liver, intestines, and skeletal muscle. A high resolving power mass spectrometer was utilized for accurate mass measurements, which permitted the use of open-source, web-based tools for MS1 annotations including METASPACE and METLIN. Whole-body MSI with IR-MALDESI allowed for broad lipid coverage with high spatial resolution, illustrating the potential of this technique for studying lipid-related diseases using zebrafish as a model organism.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Crioultramicrotomía / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Lípidos Límite: Animals Idioma: En Revista: J Am Soc Mass Spectrom Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Crioultramicrotomía / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Lípidos Límite: Animals Idioma: En Revista: J Am Soc Mass Spectrom Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos