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Isolation, purification and characterization of naturally derived Crocetin beta-d-glucosyl ester from Crocus sativus L. against breast cancer and its binding chemistry with ER-alpha/HDAC2.
Mir, Mudasir A; Ganai, Shabir Ahmad; Mansoor, Sheikh; Jan, Sumira; Mani, P; Masoodi, Khalid Z; Amin, Henna; Rehman, Muneeb U; Ahmad, Parvaiz.
Afiliación
  • Mir MA; Division of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar 190025, Jammu and Kashmir, India.
  • Ganai SA; Division of Basic Sciences and Humanities, Faculty of Agriculture, SKUAST-Kashmir, Wadura, Sopore 193201, Jammu & Kashmir, India.
  • Mansoor S; Division of Biochemistry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu 180009, J&K, India.
  • Jan S; Division of Basic Science and Humanities, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar 190025, Jammu and Kashmir, India.
  • Mani P; Department of Biotechnology, Annai College of Arts & Science, Kumbakonam, Tamil Nadu 612503, India.
  • Masoodi KZ; Division of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar 190025, Jammu and Kashmir, India.
  • Amin H; Department of Pharmaceutical Sciences, Faculty of Applied Science and Technology, University of Kashmir, Srinagar, J&K 190006, India.
  • Rehman MU; Department of Clinical Pharmacy, College of Pharmacy, King Saudi University, P. O. Box. 2460, Riyadh 11451, Saudi Arabia.
  • Ahmad P; Botany and Microbiology Department, College of Science, King Saudi University, P. O. Box. 2460, Riyadh 11451, Saudi Arabia.
Saudi J Biol Sci ; 27(3): 975-984, 2020 Mar.
Article en En | MEDLINE | ID: mdl-32127777
Saffron plant (Crocus sativus L.) is being used as a source of saffron spice and medicine to cure or prevent different types of diseases including cancers. We report the isolation, characterization of bioactive small molecule ([crocetin (ß-d-glucosyl) ester] from the leaf biowastes of saffron plant of Kashmir, India. MTTC assay and Bio-autography aided approach were used to assess anti-oxidant activity and anti-cancer properties of crocin (s) against DPPH free radical and breast cancer cell line respectively. Crocetin beta-d-glucosyl ester restrained proliferation of human breast adeno-carcinoma cell model (MCF-7) without significantly affecting normal cell line (L-6). Further studies involving molecular mechanics generalized born surface area and molecular docking showed that crocetin beta-d-glucosyl ester exhibits strong affinity for estrogen receptor alpha and histone deacetylase 2 (crucial receptors involved in breast cancer signalling) as evidenced by the negative docking score and binding free energy (BFE) values. Therefore, crocetin beta-d-glucosyl ester from Crocus sativus biowastes showed antiproliferative effect possibly by inhibiting estrogen receptor alpha and HDAC2 mediated signalling cascade.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Saudi J Biol Sci Año: 2020 Tipo del documento: Article País de afiliación: India Pais de publicación: Arabia Saudita
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Saudi J Biol Sci Año: 2020 Tipo del documento: Article País de afiliación: India Pais de publicación: Arabia Saudita