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All-trans retinoic acid enhances the effect of Fra-1 to inhibit cell proliferation and metabolism in cervical cancer.
Dou, Yingyu; Huang, Dongqing; Zeng, Xiangyang; Zhou, Yanhong; Jiang, Xiaoyan; Yue, Chunxue; He, Junyu; Xiao, Songshu.
Afiliación
  • Dou Y; Department of Gynecology and Obstetrics, The Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan, China.
  • Huang D; Department of Gynecology and Obstetrics, The Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan, China.
  • Zeng X; Department of Gynecology and Obstetrics, The Second Hospital of Zhuzhou, Zhuzhou, 412000, Hunan, China.
  • Zhou Y; Department of Gynecology and Obstetrics, The Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan, China.
  • Jiang X; The Cancer Research Institute, Central South University, Changsha, Hunan, China.
  • Yue C; Department of Gynecology and Obstetrics, The Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan, China.
  • He J; The Cancer Research Institute, Central South University, Changsha, Hunan, China.
  • Xiao S; The Cancer Research Institute, Central South University, Changsha, Hunan, China.
Biotechnol Lett ; 42(6): 1051-1060, 2020 Jun.
Article en En | MEDLINE | ID: mdl-32124141
OBJECTIVES: This study on all-trans retinoic acid was designed to explore its effect on the ability of Fra-1 to cervical cancer cell development. The results show that all-trans retinoic acid enhances the effect of Fra-1 on inhibiting cervical cancer proliferation and the glucose consumption, its effect on the loss of mitochondrial membrane potential, on the decreasing of lactic acid as well as ATP, and also influences the expression of MDM2/P53/P21 and LDHA. RESULTS: The results show that the expression of Fra-1 is higher in all-trans retinoic acid-treated cervical cancer. Flow cytometry and kit detection show that all-trans retinoic acid can enhance the ability of Fra-1 to lose the mitochondrial membrane potential, inhibit the glucose consumption and the production of lactic acid as well as ATP. CCK8 and colony formation assays indicate that all-trans retinoic acid enhances the ability of Fra-1 to inhibit cell proliferation. In addition, through Western blot analysis, it was determined that P53 and P21 were up-regulated, and MDM2 and LDHA were down-regulated. CONCLUSION: The overall results of the study strongly suggest that all-trans retinoic acid enhances the effect of Fra-1 on inhibiting cervical cancer proliferation and metabolism in vitro, and also influences the expression of MDM2/P53/P21 and LDHA.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tretinoina / Neoplasias del Cuello Uterino / Proteínas Proto-Oncogénicas c-fos / Proliferación Celular Límite: Female / Humans Idioma: En Revista: Biotechnol Lett Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tretinoina / Neoplasias del Cuello Uterino / Proteínas Proto-Oncogénicas c-fos / Proliferación Celular Límite: Female / Humans Idioma: En Revista: Biotechnol Lett Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos