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Development and characterization of standard reagents for cell-based prepandemic influenza vaccine products.
Lai, Chia-Chun; Weng, Tsai-Chuan; Chen, Po-Ling; Tseng, Yu-Fen; Lin, Chun-Yang; Chia, Min-Yuan; Sung, Wang-Chou; Lee, Min-Shi; Hu, Alan Yung-Chih.
Afiliación
  • Lai CC; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Zhunan, Taiwan.
  • Weng TC; College of Life Science, National Tsing Hua University , Hsinchu, Taiwan.
  • Chen PL; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Zhunan, Taiwan.
  • Tseng YF; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Zhunan, Taiwan.
  • Lin CY; College of Life Science, National Tsing Hua University , Hsinchu, Taiwan.
  • Chia MY; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Zhunan, Taiwan.
  • Sung WC; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Zhunan, Taiwan.
  • Lee MS; Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University , Taichung, Taiwan.
  • Hu AY; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Zhunan, Taiwan.
Hum Vaccin Immunother ; 16(9): 2245-2251, 2020 09 01.
Article en En | MEDLINE | ID: mdl-32118516
Outbreaks of infection by novel avian influenza virus strains in humans cause public health issues worldwide, and the development of vaccines against such novel strains is the most effective method for the prevention of these virus outbreaks. All types of vaccines must be tested for potency before use; thus, quantitative potency assays are needed for influenza vaccines. The single radial immunodiffusion (SRID) assay is considered the gold standard for quantification of influenza virus antigens, and the SRID reference reagents are essential for the determination of vaccine potency. However, it remains debatable whether reference reagents derived from egg-based vaccine platforms can be used to precisely quantify non-egg-derived vaccines; thus, influenza vaccine production using cell-based platforms has attracted increasing attention. To evaluate the utility of reference reagents derived from a cell-based influenza vaccine platform, we prepared cell-based reference reagents from MDCK cell-grown viruses and compared them with egg-derived reference reagents. A primary liquid standard (PLS) was purified from cell-derived candidate influenza vaccine viruses, and hemagglutinin (HA) antigen content was determined by a densitometric method. The produced PLS could be stored at 4°C for more than 10 months. We also established a simple HA protein purification method for goat antiserum preparation, and the performance of the resulting antiserum was compared to that of standard reagents obtained using different production platforms. The results of this study indicate that these reference reagents can be used for both cell-based and egg-based production platforms and that the differences between these two types of platforms are negligible.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacunas contra la Influenza / Gripe Humana Límite: Animals Idioma: En Revista: Hum Vaccin Immunother Año: 2020 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacunas contra la Influenza / Gripe Humana Límite: Animals Idioma: En Revista: Hum Vaccin Immunother Año: 2020 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos