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Efficacy of soil-borne Enterobacter sp. for carbofuran degradation: HPLC quantitation of degradation rate.
Ekram, Md Akhtar-E; Sarker, Indrani; Rahi, Md Sifat; Rahman, Md Atikur; Saha, Ananda K; Reza, Md Abu.
Afiliación
  • Ekram MA; Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, Bangladesh.
  • Sarker I; Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, Bangladesh.
  • Rahi MS; Department of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore, Bangladesh.
  • Rahman MA; Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, Bangladesh.
  • Saha AK; Department of Zoology, University of Rajshahi, Rajshahi, Bangladesh.
  • Reza MA; Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, Bangladesh.
J Basic Microbiol ; 60(5): 390-399, 2020 May.
Article en En | MEDLINE | ID: mdl-32115726
Excessive use of pesticides in agricultural fields is a matter of great concern for living beings as well as the environment across the world, in particular, the third world countries. Therefore, there is an urgent need to find out an effective way to degrade these hazardous chemicals from the soil in an environment-friendly way. In the current project, a bacterial species were isolated through enrichment culture from carbofuran-supplemented rice-field soil and identified as a carbofuran degrader. The rate of carbofuran degradation by this bacterial species was evaluated using reverse-phase high-performance liquid chromatography (RP-HPLC), which confirmed the ability to utilize as a carbon source up to 4 µg/ml of 99% technical grade carbofuran. The morphological, physiological, biochemical characteristics and phylogenetic analysis of the 16S rRNA sequence showed that this strain belongs to the genus of Enterobacter sp. (sequence accession number LC368285 in DDBJ), and the optimum growth condition for the isolated strain was 37°C at pH 7.0. Moreover, an antibiotic sensitivity test showed that it was susceptible to azithromycin, penicillin, ceftazidime, ciprofloxacin, and gentamycin, and the minimal inhibitory concentration value of gentamycin was 400 µg/ml against the bacteria. It shows beyond doubt from the RP-HPLC quantification that the isolated bacterium has the ability to detoxify carbofuran (99% pure). Finally, the obtained results imply that the isolated strain of Enterobacter can be used as a potential and effective carbofuran degrader for bioremediation of contaminated sites through bioaugmentation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbiología del Suelo / Contaminantes del Suelo / Carbofurano / Enterobacter / Insecticidas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Basic Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Bangladesh Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbiología del Suelo / Contaminantes del Suelo / Carbofurano / Enterobacter / Insecticidas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Basic Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Bangladesh Pais de publicación: Alemania