[The viability of rat amd mouse embryos in low-temperature preservation]. / Vyzhivaemost' émbrionov krys i myshei pri nizkotemperaturnoi konservatsii.
Ontogenez
; 19(5): 491-4, 1988.
Article
en Ru
| MEDLINE
| ID: mdl-3211453
Embryos of the laboratory rats and mice at the two-cell stage were frozen in different media with 1.5 M DMSO at a rate of 0.5-0.7 degrees/min to -40 degrees and the transferred into liquid nitrogen. Temperature of the crystallization initiation was -6 degrees. After thawed embryos were transplanted to foster mothers, these latter gave birth to normal offspring. The best results (38% of the total number of transplanted embryos in mice and 55% in rats) were obtained when using modified Whitten's medium for cryopreservation. Survival of the rat embryos in the NT-6 medium fell to 36% due, supposedly, to changes in pH accompanying the decrease in temperature. These results allow us to recommend modified Whitten's medium and the developed conditions of freezing-thawing for cryopreservation of the rat and mouse embryos.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Conservación de Tejido
/
Supervivencia Tisular
/
Embrión de Mamíferos
Límite:
Animals
Idioma:
Ru
Revista:
Ontogenez
Año:
1988
Tipo del documento:
Article
Pais de publicación:
Rusia