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Variant analysis of HPD genes from two families showing elevated tyrosine upon newborn screening by tandem mass spectrometry (MS/MS).
Zhao, Dehua; Tian, Yuan; Li, Xiaole; Ni, Min; Zhu, Xinyun; Jia, Liting.
Afiliación
  • Zhao D; Screening Center, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
  • Tian Y; Clinical Laboratory, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
  • Li X; Screening Center, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
  • Ni M; Screening Center, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
  • Zhu X; Screening Center, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
  • Jia L; Screening Center, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou450052, China, E-mail:jialt@163.com.
J Pediatr Endocrinol Metab ; 33(4): 563-567, 2020 Apr 28.
Article en En | MEDLINE | ID: mdl-32109208
Background Alterations in the structure and activity of 4-hydroxyphenylpyruvate dioxygenase (HPD) are causally related to two different metabolic disorders: recessively inherited tyrosinemia type III and dominantly inherited hawkinsinuria. The aim of this study was to provide a new perspective for the clinical understanding of the pathogenesis of tyrosinemia type III or hawkinsinuria. Case presentation A full-term newborn baby born after a safe pregnancy and childbirth with a birth weight of 3200 g and another full-term baby born after a safe pregnancy and childbirth with a birth weight of 2800 g are reported and analysed. DNA extraction, next-generation sequencing, bioinformatics analysis, Sanger sequencing and biochemical analysis were performed. One patient with a heterozygous HPD gene (NM_002150.2) c.460G > A mutation and one patient with a heterozygous HPD gene (NM_002150.2) c.248delG mutation showing elevated tyrosine levels upon newborn screening by tandem mass spectrometry (MS/MS) are reported. Conclusions The HPD gene may not be a strictly autosomal recessive pathogenic gene, which provides a new perspective for the clinical understanding of the pathogenesis of tyrosinemia type III or hawkinsinuria.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tirosina / Tamizaje Neonatal / Tirosinemias / Oxigenasas de Función Mixta / 4-Hidroxifenilpiruvato Dioxigenasa / Mutación Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Female / Humans / Male / Newborn Idioma: En Revista: J Pediatr Endocrinol Metab Asunto de la revista: ENDOCRINOLOGIA / PEDIATRIA Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tirosina / Tamizaje Neonatal / Tirosinemias / Oxigenasas de Función Mixta / 4-Hidroxifenilpiruvato Dioxigenasa / Mutación Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Female / Humans / Male / Newborn Idioma: En Revista: J Pediatr Endocrinol Metab Asunto de la revista: ENDOCRINOLOGIA / PEDIATRIA Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania