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A co-culture nanofibre scaffold model of neural cell degeneration in relevance to Parkinson's disease.
Chemmarappally, Joseph M; Pegram, Henry C N; Abeywickrama, Neranga; Fornari, Enzo; Hargreaves, Alan J; De Girolamo, Luigi A; Stevens, Bob.
Afiliación
  • Chemmarappally JM; Innovations in Surfaces, Materials and Related Technologies (iSMART), College of Science and Technology, Nottingham Trent University, Clifton, NG11 8NS, UK.
  • Pegram HCN; Interdisciplinary Biomedical Research Centre (IBRC), College of Science and Technology, Nottingham Trent University, Clifton, NG11 8NS, UK.
  • Abeywickrama N; Innovations in Surfaces, Materials and Related Technologies (iSMART), College of Science and Technology, Nottingham Trent University, Clifton, NG11 8NS, UK.
  • Fornari E; Interdisciplinary Biomedical Research Centre (IBRC), College of Science and Technology, Nottingham Trent University, Clifton, NG11 8NS, UK.
  • Hargreaves AJ; Innovations in Surfaces, Materials and Related Technologies (iSMART), College of Science and Technology, Nottingham Trent University, Clifton, NG11 8NS, UK.
  • De Girolamo LA; Innovations in Surfaces, Materials and Related Technologies (iSMART), College of Science and Technology, Nottingham Trent University, Clifton, NG11 8NS, UK.
  • Stevens B; Interdisciplinary Biomedical Research Centre (IBRC), College of Science and Technology, Nottingham Trent University, Clifton, NG11 8NS, UK.
Sci Rep ; 10(1): 2767, 2020 02 17.
Article en En | MEDLINE | ID: mdl-32066745
Current therapeutic strategies for Parkinson's disease (PD) aim to delay progression or replace damaged neurons by restoring the original neuronal structures. The poor regenerative capacity of neural tissue highlights the need for the development of cellular environments to model the pathogenesis of PD. In the current work, we have characterised the growth, survival and response to PD mimetics of human SH-SY5Y neuroblastoma and U-87MG glioblastoma cell lines cultured on polyacrylonitrile (PAN) and Jeffamine® doped polyacrylonitrile (PJ) nano-scaffolds. Differentiation induced by a range of agents was evaluated by immunoassays of neural protein biomarkers. PAN and PJ nanofibre scaffolds provided suitable three-dimensional (3D) environment to support the growth, differentiation and network formation of dopaminergic neuron- and astrocyte-like cell populations, respectively. The scaffolds selectively supported the survival and differentiation of both cell populations with prolonged neuronal survival when exposed to PD mimetics in the presence of astrocytes in a co-culture model. Such 3D nanoscaffold-based assays could aid our understanding of the molecular basis of PD mimetic-induced Parkinsonism and the discovery of neuroprotective agents.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedad de Parkinson / Resinas Acrílicas / Fármacos Neuroprotectores / Degeneración Nerviosa Límite: Humans Idioma: En Revista: Sci Rep Año: 2020 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedad de Parkinson / Resinas Acrílicas / Fármacos Neuroprotectores / Degeneración Nerviosa Límite: Humans Idioma: En Revista: Sci Rep Año: 2020 Tipo del documento: Article Pais de publicación: Reino Unido