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Photosynthesis is sensitive to nitric oxide and respiration sensitive to hydrogen peroxide: Studies with pea mesophyll protoplasts.
Sunil, Bobba; Rajsheel, Pidakala; Aswani, Vetcha; Bapatla, Ramesh B; Talla, Sai K; Raghavendra, Agepati S.
Afiliación
  • Sunil B; Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
  • Rajsheel P; Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
  • Aswani V; Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
  • Bapatla RB; Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
  • Talla SK; Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
  • Raghavendra AS; Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India. Electronic address: asrsl@uohyd.ernet.in.
J Plant Physiol ; 246-247: 153133, 2020.
Article en En | MEDLINE | ID: mdl-32065920
Reports on the effect of nitric oxide (NO) or reactive oxygen species (ROS) on photosynthesis and respiration in leaf tissues are intriguing; therefore, the effects of exogenous addition of sodium nitroprusside (SNP, releases NO) or H2O2 on the photosynthetic O2 evolution and respiratory O2 uptake by mesophyll protoplasts in pea (Pisum sativum) were evaluated in the present study. Low concentrations of SNP or H2O2 were used to minimize nonspecific effects. The effects of NO or H2O2 on respiration and photosynthesis were different. The presence of NO decreased the rate of photosynthesis but caused a marginal stimulation of dark respiration. Conversely, externally administered H2O2 drastically decreased the rate of respiration but only slightly decreased photosynthesis. The PS I activity was more sensitive to NO than PS II. On the other hand, 100 µM H2O2 had no effect on the photochemical reactions of either PS I or PS II. The sensitivity of photosynthesis to antimycin A or SHAM (reflecting the interplay between chloroplasts and mitochondria) was not affected by NO. By contrast, H2O2 markedly decreased the sensitivity of photosynthesis to antimycin A and SHAM. It can be concluded that chloroplasts are the primary targets of NO, while mitochondria are the primary targets of ROS in plant cells. We propose that H2O2 can be an important signal to modulate the crosstalk between chloroplasts and mitochondria.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fotosíntesis / Nitroprusiato / Especies Reactivas de Oxígeno / Pisum sativum / Peróxido de Hidrógeno / Óxido Nítrico Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Plant Physiol Asunto de la revista: BOTANICA Año: 2020 Tipo del documento: Article País de afiliación: India Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fotosíntesis / Nitroprusiato / Especies Reactivas de Oxígeno / Pisum sativum / Peróxido de Hidrógeno / Óxido Nítrico Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Plant Physiol Asunto de la revista: BOTANICA Año: 2020 Tipo del documento: Article País de afiliación: India Pais de publicación: Alemania