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The insect antimicrobial peptide cecropin A disrupts uropathogenic Escherichia coli biofilms.
Kalsy, Miriam; Tonk, Miray; Hardt, Martin; Dobrindt, Ulrich; Zdybicka-Barabas, Agnieszka; Cytrynska, Malgorzata; Vilcinskas, Andreas; Mukherjee, Krishnendu.
Afiliación
  • Kalsy M; Fraunhofer Institute for Molecular Biology and Applied Ecology, Department of Bioresources, 35394, Giessen, Germany.
  • Tonk M; Institute for Insect Biotechnology, Justus Liebig University, 35392, Giessen, Germany.
  • Hardt M; LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), 60325, Frankfurt, Germany.
  • Dobrindt U; Imaging Unit, Biomedical Research Center Seltersberg (BFS), Justus Liebig University, 35392, Giessen, Germany.
  • Zdybicka-Barabas A; Institute of Hygiene, University of Muenster, 48149, Muenster, Germany.
  • Cytrynska M; Department of Immunobiology, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, Lublin, Poland.
  • Vilcinskas A; Department of Immunobiology, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, Lublin, Poland.
  • Mukherjee K; Fraunhofer Institute for Molecular Biology and Applied Ecology, Department of Bioresources, 35394, Giessen, Germany. Andreas.Vilcinskas@agrar.uni-giessen.de.
NPJ Biofilms Microbiomes ; 6(1): 6, 2020 02 12.
Article en En | MEDLINE | ID: mdl-32051417
Current antibiotics cannot eradicate uropathogenic Escherichia coli (UPEC) biofilms, leading to recurrent urinary tract infections. Here, we show that the insect antimicrobial peptide cecropin A (CecA) can destroy planktonic and sessile biofilm-forming UPEC cells, either alone or when combined with the antibiotic nalidixic acid (NAL), synergistically clearing infection in vivo without off-target cytotoxicity. The multi-target mechanism of action involves outer membrane permeabilization followed by biofilm disruption triggered by the inhibition of efflux pump activity and interactions with extracellular and intracellular nucleic acids. These diverse targets ensure that resistance to the CecA + NAL combination emerges slowly. The antimicrobial mechanisms of CecA, thus, extend beyond pore-forming activity to include an unanticipated biofilm-eradication process, offering an alternative approach to combat antibiotic-resistant UPEC infections.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido Nalidíxico / Biopelículas / Péptidos Catiónicos Antimicrobianos / Infecciones por Escherichia coli / Proteínas Citotóxicas Formadoras de Poros / Escherichia coli Uropatógena Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: NPJ Biofilms Microbiomes Año: 2020 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido Nalidíxico / Biopelículas / Péptidos Catiónicos Antimicrobianos / Infecciones por Escherichia coli / Proteínas Citotóxicas Formadoras de Poros / Escherichia coli Uropatógena Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: NPJ Biofilms Microbiomes Año: 2020 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos