Targeted editing of transcriptional activator MXR1 on the Pichia pastoris genome using CRISPR/Cas9 technology.

Hou, Chenglin; Yang, Yankun; Xing, Yan; Zhan, Chunjun; Liu, Guoqiang; Liu, Xiuxia; Liu, Chunli; Zhan, Jinling; Xu, Dinghua; Bai, Zhonghu

Hou, Chenglin; Yang, Yankun; Xing, Yan; Zhan, Chunjun; Liu, Guoqiang; Liu, Xiuxia; Liu, Chunli; Zhan, Jinling; Xu, Dinghua; Bai, Zhonghu.

Afiliación

Hou C; The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Yang Y; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Xing Y; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China.
Zhan C; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Liu G; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China.
Liu X; The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Liu C; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Zhan J; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China.
Xu D; The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Bai Z; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.

Yeast ; 37(4): 305-312, 2020 04.

Article en En | MEDLINE | ID: mdl-32050051

Asunto(s)

Sistemas CRISPR-Cas/genética; Proteínas Fúngicas/genética; Pichia/genética; Secuencia de Bases; Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas; Medios de Cultivo/química; Proteínas Fúngicas/metabolismo; Edición Génica; Regulación Fúngica de la Expresión Génica; Metanol/metabolismo; Pichia/metabolismo; Plásmidos/genética; Plásmidos/metabolismo; Regiones Promotoras Genéticas; ARN Guía de Kinetoplastida; Factores de Transcripción

Palabras clave

CRISPR/Cas9 systems; Pichia pastoris; gene expression regulation; phosphorylation site; target gene editing; transcriptional activator Mxr1

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pichia / Proteínas Fúngicas / Sistemas CRISPR-Cas Idioma: En Revista: Yeast Asunto de la revista: MICROBIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido

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