Your browser doesn't support javascript.
loading
Towards Understanding Therapeutic Failures in Masquelet Surgery: First Evidence that Defective Induced Membrane Properties are Associated with Clinical Failures.
Durand, Marjorie; Barbier, Laure; Mathieu, Laurent; Poyot, Thomas; Demoures, Thomas; Souraud, Jean-Baptiste; Masquelet, Alain-Charles; Collombet, Jean-Marc.
Afiliación
  • Durand M; French Military Biomedical Research Institute (IRBA), BP73, 91220 Brétigny-sur-Orge, France.
  • Barbier L; French Military Biomedical Research Institute (IRBA), BP73, 91220 Brétigny-sur-Orge, France.
  • Mathieu L; Percy Military Hospital, 101 avenue Henri Barbusse 92140 Clamart, France.
  • Poyot T; French Military Biomedical Research Institute (IRBA), BP73, 91220 Brétigny-sur-Orge, France.
  • Demoures T; Bégin Military Hospital, 69 avenue de Paris, 94160 Saint-Mandé, France.
  • Souraud JB; Bégin Military Hospital, 69 avenue de Paris, 94160 Saint-Mandé, France.
  • Masquelet AC; Saint-Antoine Hospital, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France.
  • Collombet JM; French Military Biomedical Research Institute (IRBA), BP73, 91220 Brétigny-sur-Orge, France.
J Clin Med ; 9(2)2020 Feb 06.
Article en En | MEDLINE | ID: mdl-32041238
The two-stage Masquelet induced-membrane technique (IMT) consists of cement spacer-driven membrane induction followed by an autologous cancellous bone implantation in this membrane to promote large bone defect repairs. For the first time, this study aims at correlating IMT failures with physiological alterations of the induced membrane (IM) in patients. For this purpose, we compared various histological, immunohistochemical and gene expression parameters obtained from IM collected in patients categorized lately as successfully (Responders; n = 8) or unsuccessfully (Non-responders; n = 3) treated with the Masquelet technique (6 month clinical and radiologic post-surgery follow-up). While angiogenesis or macrophage distribution pattern remained unmodified in non-responder IM as compared to responder IM, we evidenced an absence of mesenchymal stem cells and reduced density of fibroblast-like cells in non-responder IM. Furthermore, non-responder IM exhibited altered extracellular matrix (ECM) remodeling parameters such as a lower expression ratio of metalloproteinase-9 (MMP-9)/tissue inhibitor of metalloproteinases (TIMP-1) mRNA as well as an important collagen overexpression as shown by picrosirius red staining. In summary, this study is the first to report evidence that IMT failure can be related to defective IM properties while underlining the importance of ECM remodeling parameters, particularly the MMP-9/TIMP-1 gene expression ratio, as early predictive biomarkers of the IMT outcome regardless of the type of bone, fracture or patient characteristics.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies / Risk_factors_studies Idioma: En Revista: J Clin Med Año: 2020 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies / Risk_factors_studies Idioma: En Revista: J Clin Med Año: 2020 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Suiza