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Increased endogenous dopamine prevents myopia in mice.
Landis, E G; Chrenek, M A; Chakraborty, R; Strickland, R; Bergen, M; Yang, V; Iuvone, P M; Pardue, M T.
Afiliación
  • Landis EG; Neuroscience, Emory University, Atlanta, GA, USA; Atlanta Veterans Affairs Healthcare System, Decatur, GA, USA.
  • Chrenek MA; Ophthalmology, Emory University, Atlanta, GA, USA.
  • Chakraborty R; Ophthalmology, Emory University, Atlanta, GA, USA.
  • Strickland R; Neuroscience, Emory University, Atlanta, GA, USA.
  • Bergen M; Ophthalmology, Emory University, Atlanta, GA, USA.
  • Yang V; Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
  • Iuvone PM; Neuroscience, Emory University, Atlanta, GA, USA; Ophthalmology, Emory University, Atlanta, GA, USA.
  • Pardue MT; Neuroscience, Emory University, Atlanta, GA, USA; Ophthalmology, Emory University, Atlanta, GA, USA; Atlanta Veterans Affairs Healthcare System, Decatur, GA, USA; Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA. Electronic address: machelle.pardue@bme.gatech.edu.
Exp Eye Res ; 193: 107956, 2020 04.
Article en En | MEDLINE | ID: mdl-32032629
Experimental evidence suggests that dopamine (DA) modulates refractive eye growth. We evaluated whether increasing endogenous DA activity using pharmacological or genetic approaches decreased myopia susceptibility in mice. First, we assessed the effects of systemic L-3,4-dihydroxyphenylalanine (L-DOPA) injections on form deprivation myopia (FDM) in C57BL/6 J (WTC57) mice. WTC57 mice received daily systemic injections of L-DOPA (n = 11), L-DOPA + ascorbic acid (AA, n = 22), AA (n = 20), or Saline (n = 16). Second, we tested transgenic mice with increased or decreased expression of vesicular monoamine transporter 2 (VMAT2HI, n = 22; WTHI, n = 18; VMAT2LO, n = 18; or WTLO, n = 9) under normal and form deprivation conditions. VMAT2 packages DA into vesicles, affecting DA release. At post-natal day 28 (P28), monocular FD was induced in each genotype. Weekly measurements of refractive error, corneal curvature, and ocular biometry were performed until P42 or P49. WTC57 mice exposed to FD developed a significant myopic shift (treated-contralateral eye) in AA (-3.27 ± 0.73D) or saline (-3.71 ± 0.80D) treated groups that was significantly attenuated by L-DOPA (-0.73 ± 0.90D, p = 0.0002) or L-DOPA + AA (-0.11 ± 0.46D, p = 0.0103). The VMAT2LO mice, with under-expression of VMAT2, were most susceptible to FDM. VMAT2LO mice developed significant myopic shifts to FD after one week compared to VMAT2HI and WT mice (VMAT2LO: -5.48 ± 0.54D; VMAT2HI: -0.52 ± 0.92D, p < 0.05; WT: -2.13 ± 0.78D, p < 0.05; ungoggled control: -0.22 ± 0.24D, p < 0.001). These results indicate that endogenously increasing DA synthesis and release by genetic and pharmacological methods prevents FDM in mice.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Refracción Ocular / Retina / Dopamina / Levodopa / Miopía Límite: Animals Idioma: En Revista: Exp Eye Res Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Refracción Ocular / Retina / Dopamina / Levodopa / Miopía Límite: Animals Idioma: En Revista: Exp Eye Res Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido