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AAV-Mediated Gene Delivery to 3D Retinal Organoids Derived from Human Induced Pluripotent Stem Cells.
Garita-Hernandez, Marcela; Routet, Fiona; Guibbal, Laure; Khabou, Hanen; Toualbi, Lyes; Riancho, Luisa; Reichman, Sacha; Duebel, Jens; Sahel, Jose-Alain; Goureau, Olivier; Dalkara, Deniz.
Afiliación
  • Garita-Hernandez M; Institut de la Vision, Sorbonne Université, INSERM, CNRS, 75012 Paris, France.
  • Routet F; Institut de Neurosciences de Montpellier, Université de Montpellier, INSERM, 34090 Montpellier, France.
  • Guibbal L; Institut de la Vision, Sorbonne Université, INSERM, CNRS, 75012 Paris, France.
  • Khabou H; Institut de la Vision, Sorbonne Université, INSERM, CNRS, 75012 Paris, France.
  • Toualbi L; Institut de la Vision, Sorbonne Université, INSERM, CNRS, 75012 Paris, France.
  • Riancho L; Institut de la Vision, Sorbonne Université, INSERM, CNRS, 75012 Paris, France.
  • Reichman S; Institut de la Vision, Sorbonne Université, INSERM, CNRS, 75012 Paris, France.
  • Duebel J; Institut de la Vision, Sorbonne Université, INSERM, CNRS, 75012 Paris, France.
  • Sahel JA; Institut de la Vision, Sorbonne Université, INSERM, CNRS, 75012 Paris, France.
  • Goureau O; Department of Ophthalmology, University Medical Center Göttingen, 37075 Göttingen, Germany.
  • Dalkara D; Institut de la Vision, Sorbonne Université, INSERM, CNRS, 75012 Paris, France.
Int J Mol Sci ; 21(3)2020 Feb 03.
Article en En | MEDLINE | ID: mdl-32028585
Human induced pluripotent stem cells (hiPSCs) promise a great number of future applications to investigate retinal development, pathophysiology and cell therapies for retinal degenerative diseases. Specific approaches to genetically modulate hiPSC would be valuable for all of these applications. Vectors based on adeno-associated virus (AAV) have shown the ability for gene delivery to retinal organoids derived from hiPSCs. Thus far, little work has been carried out to investigate mechanisms of AAV-mediated gene delivery and the potential advantages of engineered AAVs to genetically modify retinal organoids. In this study, we compared the early transduction efficiency of several recombinant and engineered AAVs in hiPSC-derived RPE cells and retinal organoids in relation to the availability of their cell-surface receptors and as a function of time. The genetic variant AAV2-7m8 had a superior transduction efficiency when applied at day 44 of differentiation on retinal organoids and provided long-lasting expressions for at least 4 weeks after infection without compromising cell viability. All of the capsids we tested transduced the hiPSC-RPE cells, with the AAV2-7m8 variant being the most efficient. Transduction efficiency was correlated with the presence of primary cell-surface receptors on the hiPS-derived organoids. Our study explores some of the mechanisms of cell attachment of AAVs and reports long-term gene expression resulting from gene delivery in retinal organoids.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Retina / Organoides / Técnicas de Transferencia de Gen / Dependovirus / Proteínas Fluorescentes Verdes / Células Madre Pluripotentes Inducidas / Vectores Genéticos Límite: Animals / Humans Idioma: En Revista: Int J Mol Sci Año: 2020 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Retina / Organoides / Técnicas de Transferencia de Gen / Dependovirus / Proteínas Fluorescentes Verdes / Células Madre Pluripotentes Inducidas / Vectores Genéticos Límite: Animals / Humans Idioma: En Revista: Int J Mol Sci Año: 2020 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Suiza