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Paraburkholderia madseniana sp. nov., a phenolic acid-degrading bacterium isolated from acidic forest soil.
Wilhelm, Roland C; Murphy, Sean J L; Feriancek, Nicole M; Karasz, David C; DeRito, Christopher M; Newman, Jeffrey D; Buckley, Daniel H.
Afiliación
  • Wilhelm RC; School of Integrative Plant Sciences, Bradfield Hall, Cornell University, Ithaca, NY, 14853, USA.
  • Murphy SJL; School of Integrative Plant Sciences, Bradfield Hall, Cornell University, Ithaca, NY, 14853, USA.
  • Feriancek NM; School of Integrative Plant Sciences, Bradfield Hall, Cornell University, Ithaca, NY, 14853, USA.
  • Karasz DC; School of Integrative Plant Sciences, Bradfield Hall, Cornell University, Ithaca, NY, 14853, USA.
  • DeRito CM; Department of Microbiology, Wing Hall, Cornell University, Ithaca, NY, 14853, USA.
  • Newman JD; Lycoming College, Williamsport, PA, 17701, USA.
  • Buckley DH; School of Integrative Plant Sciences, Bradfield Hall, Cornell University, Ithaca, NY, 14853, USA.
Int J Syst Evol Microbiol ; 70(3): 2137-2146, 2020 Mar.
Article en En | MEDLINE | ID: mdl-32027304
RP11T was isolated from forest soil following enrichment with 4-hydroxybenzoic acid. Cells of RP11T are aerobic, non-sporulating, exhibit swimming motility, and are rods (0.8 µm by 1.4 µm) that often occur as diplobacillus or in short chains (3-4 cells). Optimal growth on minimal media containing 4-hydroxybenzoic acid (µ=0.216 hr-1) occurred at 30 °C, pH 6.5 or 7.0 and 0% salinity. Comparative chemotaxonomic, genomic and phylogenetic analyses revealed the isolate was distinct from its closest relative type strains identified as Paraburkholderia aspalathi LMG 27731T, Paraburkholderia fungorum LMG 16225T and Paraburkholderia caffeinilytica CF1T. Strain RP11T is genetically distinct from P. aspalathi, its closest relative, in terms of 16S rRNA gene sequence similarity (98.7%), genomic average nucleotide identity (94%) and in silico DNA-DNA hybridization (56.7 %±2.8). The composition of fatty acids and substrate utilization pattern differentiated strain RP11T from its closest relatives, including growth on phthalic acid. Strain RP11T encoded the greatest number of aromatic degradation genes of all eleven closely related type strains and uniquely encoded a phthalic acid dioxygenase and paralog of the 3-hydroxybenzoate 4-monooxygenase. The only ubiquinone detected in strain RP11T was Q-8, and the major cellular fatty acids were C16 : 0, 3OH-C16 : 0, C17 : 0 cyclo, C19 : 0 cyclo ω8c, and summed feature 8 (C18 : 1 ω7c/ω6c). On the basis of this polyphasic approach, it was determined that strain RP11T represents a novel species from the genus Paraburkholderia for which the name Paraburkholderia madseniana sp. nov. is proposed. The type strain is RP11T (=DSM 110123T=LMG 31517T).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Microbiología del Suelo / Bosques / Burkholderiaceae / Hidroxibenzoatos País/Región como asunto: America do norte Idioma: En Revista: Int J Syst Evol Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Microbiología del Suelo / Bosques / Burkholderiaceae / Hidroxibenzoatos País/Región como asunto: America do norte Idioma: En Revista: Int J Syst Evol Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido