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Novel monoclonal antibodies against Australian strains of negeviruses and insights into virus structure, replication and host -restriction.
Colmant, Agathe M G; O'Brien, Caitlin A; Newton, Natalee D; Watterson, Daniel; Hardy, Joshua; Coulibaly, Fasséli; Bielefeldt-Ohmann, Helle; Warrilow, David; Huang, Bixing; Paramitha, Devina; Harrison, Jessica J; Hall, Roy A; Hobson-Peters, Jody.
Afiliación
  • Colmant AMG; Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia 4072, Queensland, Australia.
  • O'Brien CA; Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia 4072, Queensland, Australia.
  • Newton ND; Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia 4072, Queensland, Australia.
  • Watterson D; Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia 4072, Queensland, Australia.
  • Hardy J; Infection and Immunity, Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia.
  • Coulibaly F; Infection and Immunity, Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia.
  • Bielefeldt-Ohmann H; School of Veterinary Science, The University of Queensland, Queensland, Gatton, Australia.
  • Warrilow D; Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia 4072, Queensland, Australia.
  • Huang B; Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government, 39 Kessels Rd, Coopers Plains, QLD 4108, Australia.
  • Paramitha D; Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government, 39 Kessels Rd, Coopers Plains, QLD 4108, Australia.
  • Harrison JJ; Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia 4072, Queensland, Australia.
  • Hall RA; Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia 4072, Queensland, Australia.
  • Hobson-Peters J; Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia 4072, Queensland, Australia.
J Gen Virol ; 101(4): 440-452, 2020 04.
Article en En | MEDLINE | ID: mdl-32003709
We report the isolation of Australian strains of Bustos virus and Ngewotan virus, two insect-specific viruses in the newly identified taxon Negevirus, originally isolated from Southeast Asian mosquitoes. Consistent with the expected insect-specific tropism of negeviruses, these isolates of Ngewotan and Bustos viruses, alongside the Australian negevirus Castlerea virus, replicated exclusively in mosquito cells but not in vertebrate cells, even when their temperature was reduced to 34 °C. Our data confirmed the existence of two structural proteins, putatively one membrane protein forming the majority of the virus particle, and one glycoprotein forming a projection on the apex of the virions. We generated and characterized 71 monoclonal antibodies to both structural proteins of the two viruses, most of which were neutralizing. Overall, these data increase our knowledge of negevirus mechanisms of infection and replication in vitro.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virión / Replicación Viral / Proteínas Estructurales Virales / Virus de Insectos / Anticuerpos Monoclonales / Culicidae Límite: Animals País/Región como asunto: Oceania Idioma: En Revista: J Gen Virol Año: 2020 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virión / Replicación Viral / Proteínas Estructurales Virales / Virus de Insectos / Anticuerpos Monoclonales / Culicidae Límite: Animals País/Región como asunto: Oceania Idioma: En Revista: J Gen Virol Año: 2020 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido