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Metabolic Engineering of Saccharomyces cerevisiae To Overproduce Squalene.
Li, Tian; Liu, Guo-Song; Zhou, Wei; Jiang, Min; Ren, Yu-Hong; Tao, Xin-Yi; Liu, Min; Zhao, Ming; Wang, Feng-Qing; Gao, Bei; Wei, Dong-Zhi.
Afiliación
  • Li T; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology , East China University of Science and Technology , 130 Meilong Road , Shanghai 200237 , China.
  • Liu GS; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology , East China University of Science and Technology , 130 Meilong Road , Shanghai 200237 , China.
  • Zhou W; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology , East China University of Science and Technology , 130 Meilong Road , Shanghai 200237 , China.
  • Jiang M; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology , East China University of Science and Technology , 130 Meilong Road , Shanghai 200237 , China.
  • Ren YH; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology , East China University of Science and Technology , 130 Meilong Road , Shanghai 200237 , China.
  • Tao XY; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology , East China University of Science and Technology , 130 Meilong Road , Shanghai 200237 , China.
  • Liu M; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology , East China University of Science and Technology , 130 Meilong Road , Shanghai 200237 , China.
  • Zhao M; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology , East China University of Science and Technology , 130 Meilong Road , Shanghai 200237 , China.
  • Wang FQ; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology , East China University of Science and Technology , 130 Meilong Road , Shanghai 200237 , China.
  • Gao B; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology , East China University of Science and Technology , 130 Meilong Road , Shanghai 200237 , China.
  • Wei DZ; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology , East China University of Science and Technology , 130 Meilong Road , Shanghai 200237 , China.
J Agric Food Chem ; 68(7): 2132-2138, 2020 Feb 19.
Article en En | MEDLINE | ID: mdl-31989819
Squalene has wide applications in the food and pharmaceutical industries. Engineering microbes to produce squalene is a promising alternative for traditional production approaches. In this study, squalene production was enhanced to 978.24 mg/L through stepwise overexpression of the enzymes that catalyze acetyl-CoA to squalene. Subsequently, to increase the activity of HMG-CoA reductase and alleviate the high dependence on NADPH, the HMG-CoA reductase (NADH-HMGR) from Silicibacter pomeroyi, highly specific for NADH, was introduced, which increased squalene production to 1086.31 mg/L. Native ethanol dehydrogenase ADH2 and acetaldehyde dehydrogenase ADA from Dickeya zeae were further overexpressed, which enhanced the capability to utilize ethanol for squalene synthesis and endowed the engineered strain with greater adaptability to high ethanol concentrations. Finally, a remarkable squalene production of 9472 mg/L was obtained from ethanol via carbon source-controlled fed-batch fermentation. This study will greatly accelerate the process of developing microbial cell factories for squalene production.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Escualeno Idioma: En Revista: J Agric Food Chem Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Escualeno Idioma: En Revista: J Agric Food Chem Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos