Putative novel Bradyrhizobium and Phyllobacterium species isolated from root nodules of Chamaecytisus ruthenicus.

Kalita, Michal; Malek, Wanda; Coutinho, Teresa A

Kalita, Michal; Malek, Wanda; Coutinho, Teresa A.

Afiliación

Kalita M; Department of Genetics and Microbiology, M. Curie-Sklodowska University, Lublin, Poland. Electronic address: michal.kalita@umcs.pl.
Malek W; Department of Genetics and Microbiology, M. Curie-Sklodowska University, Lublin, Poland.
Coutinho TA; Centre for Microbial Ecology and Genomics, Forestry and Agricultural Biotechnology Institute, Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Pretoria, South Africa.

Syst Appl Microbiol ; 43(2): 126056, 2020 Mar.

Article en En | MEDLINE | ID: mdl-31987702

RESUMEN

In this study, the diversity and the phylogenetic relationships of bacteria isolated from root nodules of Chamaecytisus ruthenicus growing in Poland were investigated using ERIC-PCR fingerprinting and by multilocus sequence analysis (MLSA). Two major clusters comprising 13 and 3 isolates were detected which 16S rRNA gene sequencing identified as Bradyrhizobium and Phyllobacterium. The results of phylogenetic analysis of individual and concatenated atpD, gyrB and recA gene sequences showed that the studied strains may represent novel species in the genera Bradyrhizobium and Phyllobacterium. In the phylogenetic tree based on the atpD-gyrB-recA concatemers, Bradyrhizobium isolates were split into two groups closely related to Bradyrhizobium algeriense STM89T and Bradyrhizobium valentinum LmjM3T. The genus Phyllobacterium isolates formed a separate cluster close to Phyllobacterium ifriqiyense LMG27887T in the atpD-gyrB-recA phylogram. Analysis of symbiotic gene sequences (nodC, nodZ, nifD, and nifH) showed that the Bradyrhizobium isolates were most closely related to Bradyrhizobium algeriense STM89T, Bradyrhizobium valentinum LmjM3T and Bradyrhizobium retamae Ro19T belonging to symbiovar retamae. This is the first report on the occurrence of members of symbiovar retamae from outside the Mediterranean region. No symbiosis related genes were amplified from Phyllobacterium strains, which were also unable to induce nodules on C. ruthenicus roots. Based on these findings Phyllobacterium isolates can be regarded as endophytic bacteria inhabitating root nodules of C. ruthenicus.

Asunto(s)

Bradyrhizobium/clasificación; Fabaceae/microbiología; Phyllobacteriaceae/clasificación; Filogenia; Proteínas Bacterianas/genética; Bradyrhizobium/genética; Bradyrhizobium/aislamiento & purificación; ADN Bacteriano/genética; Genes Esenciales/genética; Variación Genética; Fijación del Nitrógeno/genética; Phyllobacteriaceae/genética; Phyllobacteriaceae/aislamiento & purificación; Nodulación de la Raíz de la Planta/genética; Polonia; ARN Ribosómico 16S/genética; Nódulos de las Raíces de las Plantas/microbiología; Análisis de Secuencia de ADN; Simbiosis/genética

Palabras clave

Bradyrhizobium; Chamaecytisus ruthenicus; Housekeeping genes; Nodulation genes; Phyllobacterium

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Bradyrhizobium / Phyllobacteriaceae / Fabaceae Tipo de estudio: Prognostic_studies País/Región como asunto: Europa Idioma: En Revista: Syst Appl Microbiol Año: 2020 Tipo del documento: Article Pais de publicación: Alemania

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google