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Identification and quantification of myo-inositol hexakisphosphate in complex environmental matrices using ion chromatography and high-resolution mass spectrometry in comparison to 31P NMR spectroscopy.
McIntyre, Catherine A; Arkell, Jennifer J L; Arthur, Christopher J; Lawrence, Paul G; Butts, Craig P; Lloyd, Charlotte E M; Johnes, Penny J; Evershed, Richard P.
Afiliación
  • McIntyre CA; Organic Geochemistry Unit, School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, United Kingdom.
  • Arkell JJL; Organic Geochemistry Unit, School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, United Kingdom.
  • Arthur CJ; School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, United Kingdom.
  • Lawrence PG; School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, United Kingdom.
  • Butts CP; School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, United Kingdom.
  • Lloyd CEM; Organic Geochemistry Unit, School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, United Kingdom.
  • Johnes PJ; School of Geographical Sciences, University of Bristol, University Road, Bristol, BS8 1SS, United Kingdom.
  • Evershed RP; Organic Geochemistry Unit, School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, United Kingdom. Electronic address: r.p.evershed@bristol.ac.uk.
Talanta ; 210: 120188, 2020 Apr 01.
Article en En | MEDLINE | ID: mdl-31987164
Myo-inositol hexakisphosphate, or phytic acid, (myo-IP6) is a key organic phosphorus (P) compound in soils and manures. Determinations of myo-IP6 in soils and manure extracts are frequently performed by 31P NMR spectroscopy. This approach is time-consuming in terms of both sample preparation and instrument time, with uncertainties existing in relation to accuracy of identification and quantification due to potentially interfering resonances from co-extracted P species. In contrast, ion chromatography (IC) in combination with high-resolution mass spectrometry (HRMS) negative ion, electrospray ionisation (ESI) has been shown to enable highly specific identifications of myo-IP6 isolated from complex mixtures. In this paper, IC and ESI-HRMS were applied to the identification and the quantification of myo-IP6 isolated from soils and manures using NaOH-EDTA extraction, and quantifications based on IC. ESI-HRMS analysis of eluate trapped from IC unequivocally confirmed identification of myo-IP6 from a soil extract. The ion suppression cell of the IC instrument provides isolates of the analyte free of ionic components that would interfere with ESI. The myo-IP6 was identified in the NMR by comparing spectra of extracts of soils with and without authentic myo-IP6 "spiked" prior to extraction. Comparison of quantification via standard addition in IC and NMR analysis gave good correlation (r = 0.955). IC with ESI-HRMS was found to be more sensitive, rapid and reliable for the identification and quantification of myo-IP6 with a limit of detection (LOD) of 0.7 mg kg-1 and limit of quantification (LOQ) of 2.1 mg kg-1 using IC versus > 10 mg kg-1 LOD using 31P NMR.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Talanta Año: 2020 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Talanta Año: 2020 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Países Bajos