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Burkholderia pseudomallei invades the olfactory nerve and bulb after epithelial injury in mice and causes the formation of multinucleated giant glial cells in vitro.
Walkden, Heidi; Delbaz, Ali; Nazareth, Lynn; Batzloff, Michael; Shelper, Todd; Beacham, Ifor R; Chacko, Anu; Shah, Megha; Beagley, Kenneth W; Tello Velasquez, Johana; St John, James A; Ekberg, Jenny A K.
Afiliación
  • Walkden H; Menzies Health Institute Queensland, Griffith University, Southport, Australia.
  • Delbaz A; Clem Jones Centre for Neurobiology and Stem Cell Research, Griffith University, Nathan, Australia.
  • Nazareth L; Menzies Health Institute Queensland, Griffith University, Southport, Australia.
  • Batzloff M; Clem Jones Centre for Neurobiology and Stem Cell Research, Griffith University, Nathan, Australia.
  • Shelper T; Menzies Health Institute Queensland, Griffith University, Southport, Australia.
  • Beacham IR; Clem Jones Centre for Neurobiology and Stem Cell Research, Griffith University, Nathan, Australia.
  • Chacko A; Institute for Glycomics, Griffith University, Southport, Australia.
  • Shah M; Menzies Health Institute Queensland, Griffith University, Southport, Australia.
  • Beagley KW; Clem Jones Centre for Neurobiology and Stem Cell Research, Griffith University, Nathan, Australia.
  • Tello Velasquez J; Institute for Glycomics, Griffith University, Southport, Australia.
  • St John JA; Menzies Health Institute Queensland, Griffith University, Southport, Australia.
  • Ekberg JAK; Clem Jones Centre for Neurobiology and Stem Cell Research, Griffith University, Nathan, Australia.
PLoS Negl Trop Dis ; 14(1): e0008017, 2020 01.
Article en En | MEDLINE | ID: mdl-31978058
The infectious disease melioidosis is caused by the bacterium Burkholderia pseudomallei. Melioidosis is characterised by high mortality and morbidity and can involve the central nervous system (CNS). We have previously discovered that B. pseudomallei can infect the CNS via the olfactory and trigeminal nerves in mice. We have shown that the nerve path is dependent on mouse strain, with outbred mice showing resistance to olfactory nerve infection. Damage to the nasal epithelium by environmental factors is common, and we hypothesised that injury to the olfactory epithelium may increase the vulnerability of the olfactory nerve to microbial insult. We therefore investigated this, using outbred mice that were intranasally inoculated with B. pseudomallei, with or without methimazole-induced injury to the olfactory neuroepithelium. Methimazole-mediated injury resulted in increased B. pseudomallei invasion of the olfactory epithelium, and only in pre-injured animals were bacteria found in the olfactory nerve and bulb. In vitro assays demonstrated that B. pseudomallei readily infected glial cells isolated from the olfactory and trigeminal nerves (olfactory ensheathing cells and trigeminal Schwann cells, respectively). Bacteria were degraded by some cells but persisted in other cells, which led to the formation of multinucleated giant cells (MNGCs), with olfactory ensheathing cells less likely to form MNGCs than Schwann cells. Double Cap mutant bacteria, lacking the protein BimA, did not form MNGCs. These data suggest that injuries to the olfactory epithelium expose the primary olfactory nervous system to bacterial invasion, which can then result in CNS infection with potential pathogenic consequences for the glial cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bulbo Olfatorio / Nervio Olfatorio / Burkholderia pseudomallei / Subunidad beta de la Proteína de Unión al Calcio S100 / Melioidosis Tipo de estudio: Etiology_studies Límite: Animals / Humans Idioma: En Revista: PLoS Negl Trop Dis Asunto de la revista: MEDICINA TROPICAL Año: 2020 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bulbo Olfatorio / Nervio Olfatorio / Burkholderia pseudomallei / Subunidad beta de la Proteína de Unión al Calcio S100 / Melioidosis Tipo de estudio: Etiology_studies Límite: Animals / Humans Idioma: En Revista: PLoS Negl Trop Dis Asunto de la revista: MEDICINA TROPICAL Año: 2020 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos