Ion Abundance Ratios of Qualitative Analysis by GC-MS of 4 Common Drugs ï¼Poisons.
Fa Yi Xue Za Zhi
; 35(6): 687-694, 2019 Dec.
Article
en En, Zh
| MEDLINE
| ID: mdl-31970955
ABSTRACT: Objective To investigate the maximum allowable deviation of ion abundance ratios of characteristic fragment ions in common drugs ï¼poisonsï¼ in blood by gas chromatography-mass spectrometry ï¼GC-MSï¼ method. Methods Four common drugs ï¼poisonsï¼ ï¼dichlorvos, phorate, diazepam and estazolamï¼ were detected by GC-MS full scan mode after liquid-liquid extraction in two laboratories and under three chromatographic conditions. The deviations of ion abundance ratios of the four common drugs ï¼poisonsï¼ in marked blood samples with concentrations of 0.5, 1.0, 2.0, 5.0 and 10.0 µg/mL were analyzed. At the same time, the false negative rates of ion abundance ratios were analyzed when the mass concentration was limit of detection ï¼LODï¼, 2LOD, limit of quantitation ï¼LOQï¼ and 2LOQ, and the false positive rates of ion abundance ratios were analyzed with blank blood samples. Results Under the two laboratories, four common drugs ï¼poisonsï¼ and three kinds of chromatography conditions, the differences in deviations of the ion abundance ratios of marked blood samples were not statistically significant ï¼P>0.05ï¼. More than 95% of the absolute deviations of the ion abundance ratios of the marked blood samples were within the range of ±10%, and more than 95% of the relative deviations were within the range of ±25%. In cases of low concentration ï¼concentration less than 2LOQï¼ or low signal to noise ratio ï¼3-15ï¼, the false negative rate was less than 5% and the false positive rate was 0% when the relative deviation was greater than 50%. Conclusion The absolute deviations of ion abundance ratios of four common drugs ï¼poisonsï¼ in marked blood samples are advised to have a determination range within ±10%, and the determination range of relative deviations within ±25%.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Venenos
/
Iones
/
Cromatografía de Gases y Espectrometría de Masas
Tipo de estudio:
Qualitative_research
Límite:
Humans
Idioma:
En
/
Zh
Revista:
Fa Yi Xue Za Zhi
Asunto de la revista:
JURISPRUDENCIA
Año:
2019
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China