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Identification and characterization of the glycoside hydrolase family 18 genes from the entomopathogenic fungus Isaria cicadae genome.
Peng, Yao; Wang, Lifang; Gao, Yan; Ye, Liang; Xu, Huihui; Li, Shuangjiao; Jiang, Junqi; Li, Guiting; Dang, Xiangli.
Afiliación
  • Peng Y; School of Plant Protection, Anhui Agricultural University, Hefei 230036, P.R. China.
  • Wang L; School of Horticulture, Anhui Agricultural University, Hefei 230036, P.R. China.
  • Gao Y; Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R. China.
  • Ye L; School of Plant Protection, Anhui Agricultural University, Hefei 230036, P.R. China.
  • Xu H; School of Plant Protection, Anhui Agricultural University, Hefei 230036, P.R. China.
  • Li S; School of Plant Protection, Anhui Agricultural University, Hefei 230036, P.R. China.
  • Jiang J; School of Plant Protection, Anhui Agricultural University, Hefei 230036, P.R. China.
  • Li G; School of Plant Protection, Anhui Agricultural University, Hefei 230036, P.R. China.
  • Dang X; School of Plant Protection, Anhui Agricultural University, Hefei 230036, P.R. China.
Can J Microbiol ; 66(4): 274-287, 2020 Apr.
Article en En | MEDLINE | ID: mdl-31961710
Fungal chitinases play essential roles in chitin degradation, cell wall remodeling, chitin recycling, nutrition acquisition, autolysis, and virulence. In this study, 18 genes of the glycoside hydrolase 18 (GH18) family were identified in the Isaria cicadae genome. Seventeen of the genes belonged to chitinases and one was an endo-ß-N-acetylglucosaminidase (ENGase). According to phylogenetic analysis, the 17 chitinases were designated as subgroups A (7 chitinases), B (7), and C (3). The exon-intron organizations of these genes were analyzed. The conserved regions DxxDxDxE and S/AxGG and the domains CBM1, CBM18, and CBM50 were detected in I. cicadae chitinases and ENGase. The results of analysis of expression patterns showed that genes ICchiA1, ICchiA6, ICchiB1, and ICchiB4 had high transcript levels in the different growth conditions or developmental stages. Subgroup A chitinase genes had higher transcript levels than the genes of all other chitinases. Subgroup B chitinase genes (except ICchiB7) presented higher transcript levels in chitin medium compared with other conditions. ICchiC2 and ICchiC3 were mainly transcribed in autolysis medium and in blastospores, respectively. Moreover, ICchiB1 presented higher transcript levels than genes of other chitinases. This work provides an overview of the GH18 chitinases and ENGase in I. cicadae and provides a context for the chitinolytic potential, functions, and biological controls of these enzymes of entomopathogenic fungi.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Fúngicas / Quitinasas / Genoma Fúngico / Hongos Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Can J Microbiol Año: 2020 Tipo del documento: Article Pais de publicación: Canadá
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Fúngicas / Quitinasas / Genoma Fúngico / Hongos Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Can J Microbiol Año: 2020 Tipo del documento: Article Pais de publicación: Canadá