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Epitope Tagging ChIP-Seq of DNA Binding Proteins Using CETCh-Seq.
Meadows, Sarah K; Brandsmeier, Laurel A; Newberry, Kimberly M; Betti, Michael J; Nesmith, Amy S; Mackiewicz, Mark; Partridge, E Christopher; Mendenhall, Eric M; Myers, Richard M.
Afiliación
  • Meadows SK; HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA.
  • Brandsmeier LA; HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA.
  • Newberry KM; HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA.
  • Betti MJ; University of Alabama in Huntsville, Huntsville, AL, USA.
  • Nesmith AS; HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA.
  • Mackiewicz M; HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA.
  • Partridge EC; HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA.
  • Mendenhall EM; HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA.
  • Myers RM; University of Alabama in Huntsville, Huntsville, AL, USA.
Methods Mol Biol ; 2117: 3-34, 2020.
Article en En | MEDLINE | ID: mdl-31960370
Chromatin immunoprecipitation followed by next-generation DNA sequencing (ChIP-seq) has been used to identify transcription factor (TF) binding proteins throughout the genome. Unfortunately, this approach traditionally requires commercially available, ChIP-seq grade antibodies that frequently fail to generate acceptable datasets. To obtain data for the many TFs for which there is no appropriate antibody, we recently developed a new method for performing ChIP-seq by epitope tagging endogenous TFs using CRISPR/Cas9 genome editing technology (CETCh-seq). Here, we describe our general protocol of CETCh-seq for both adherent and nonadherent cell lines using a commercially available FLAG antibody.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Epítopos Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Epítopos Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos