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Major histocompatibility complex control of NK-related allogeneic lymphocyte cytotoxicity in rats. The contributions of strong and medial transplantation antigens.
Ager, A; Fajumi, J; Sparshott, S M; Ford, W L; Butcher, G W.
Afiliación
  • Ager A; Department of Cell and Structural Biology, University of Manchester, United Kingdom.
Transplantation ; 46(5): 762-7, 1988 Nov.
Article en En | MEDLINE | ID: mdl-3194940
Allogeneic lymphocyte cytotoxicity (ALC) describes the elimination of allogeneic lymphocytes in vivo by an NK-related activity. There is evidence that ALC is demonstrable between donor and recipient when these are incompatible at MHC gene loci alone. Since ALC is a property of T cell-deficient nude rats, the role of the MHC in this rejection process needs further study. We have determined the contribution of the MHC to ALC using congenic and recombinant rats. In our analysis we have assumed that ALC involves the recognition of classic alloantigens by clonally distributed effector cells as for other examples of transplant rejection, although this is not yet proved. Strong ALC was measured between congenic rats that differed for MHC genes only. Non-MHC incompatibility alone did not elicit ALC. In the presence of MHC incompatibility the strength of ALC generated in a recipient was dependent on non-MHC genes. The PVG background generated high ALC responses whereas ALC was not measured in the DA rat. However ALC was measured in the congenic PVG-RT1avl (DA) rat. The contributions of classic class I (RT1.A), class II (RT1.B/D), and medial transplantation (RT1.C) regions of the rat MHC were determined by comparing different recombinant donors into the same recipient strain. Single region differences alone in any of these three MHC regions did not elicit full ALC. In two sets of transfers a combination of RT1.B/D and RT1.C region incompatibility was sufficient to generate a full allogeneic response. It can be concluded that the controlling element for allogeneic lymphocyte cytotoxicity is in the RT1.B/D-RT1.C region of the rat MHC.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ratas Endogámicas / Células Asesinas Naturales / Citotoxicidad Inmunológica / Antígenos de Histocompatibilidad / Isoantígenos Límite: Animals Idioma: En Revista: Transplantation Año: 1988 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ratas Endogámicas / Células Asesinas Naturales / Citotoxicidad Inmunológica / Antígenos de Histocompatibilidad / Isoantígenos Límite: Animals Idioma: En Revista: Transplantation Año: 1988 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos